50185
Anti-Mouse-IgG - Atto 647N antibody produced in goat
contains 50% glycerol as stabilizer
Synonym(s):
Atto 647N-Anti-Mouse-IgG antibody produced in goat
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About This Item
Technique(s):
immunofluorescence: 5 μg/mL
Conjugate:
Atto 647N conjugate
Application:
IF
Citations:
50
conjugate
Atto 647N conjugate
Quality Level
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
contains
50% glycerol as stabilizer
species reactivity
mouse
concentration
≥0.8 mg/mL IgG
technique(s)
immunofluorescence: 5 μg/mL
fluorescence
λex 647 nm; λem 665 nm in PBS
suitability
in accordance for fluorescence
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Related Categories
General description
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice. Atto 647N-goat anti-mouse IgG associates with mouse IgGs.
Immunogen
mouse IgG
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.Atto 647N goat anti-rabbit IgG was used as the secondary antibody for immunofluorescene at a concentration of 5μg/ml on cells fixed in 2% formaldehyde.
Physical form
Atto 647 goat anti-mouse IgG (whole molecule) is provided in unit sizes of 1 ml as 1 mg/ml solutions in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, containing 5 mM sodium azide as a preservative.
Analysis Note
unconjugated dye ≤5% of total fluorescence
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
Regulatory Information
常规特殊物品
低风险生物材料
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Zoran V Popovic et al.
Scientific reports, 7(1), 311-311 (2017-03-24)
Tissue osmolarity varies among different organs and can be considerably increased under pathologic conditions. Hyperosmolarity has been associated with altered stimulatory properties of immune cells, especially macrophages and dendritic cells. We have recently reported that dendritic cells upon exposure to
Beatriz Marcos-Ramiro et al.
The Journal of cell biology, 213(3), 385-402 (2016-05-04)
Endothelial barrier dysfunction underlies chronic inflammatory diseases. In searching for new proteins essential to the human endothelial inflammatory response, we have found that the endosomal GTPase RhoB is up-regulated in response to inflammatory cytokines and expressed in the endothelium of
Elisa Merklinger et al.
eLife, 6 (2017-07-20)
Molecule clustering is an important mechanism underlying cellular self-organization. In the cell membrane, a variety of fundamentally different mechanisms drive membrane protein clustering into nanometre-sized assemblies. To date, it is unknown whether this clustering process can be dissected into steps
J Splinter et al.
Mutagenesis, 25(3), 289-297 (2010-02-20)
The induction of localized DNA damage within a discrete nuclear volume is an important tool in DNA repair studies. Both charged particle irradiation and laser microirradiation (LMI) systems allow for such a localized damage induction, but the results obtained are
David Drutovic et al.
The EMBO journal, 39(1), e101689-e101689 (2019-10-17)
Homologous chromosome segregation during meiosis I (MI) in mammalian oocytes is carried out by the acentrosomal MI spindles. Whereas studies in human oocytes identified Ran GTPase as a crucial regulator of the MI spindle function, experiments in mouse oocytes questioned
Articles
Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.
免疫印迹（蛋白质印迹转印）是现代蛋白质组学研究中的常用技术。
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 50185-1ML-F | 04061838061997 |
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