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Merck
CN

50649

Glyoxal solution

BioReagent, ~40% in H2O (~8.8 M), Molecular Biology

Synonym(s):

Ethanedial, Oxalaldehyde

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About This Item

Linear Formula:
OHCCHO
CAS Number:
Molecular Weight:
58.04
UNSPSC Code:
12352114
NACRES:
NA.52
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
1732463
Form:
liquid
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grade

Molecular Biology

Quality Level

product line

BioReagent

form

liquid

concentration

~40% in H2O (~8.8 M)

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

refractive index

n20/D 1.409

anion traces

chloride (Cl-): ≤100 mg/kg, sulfate (SO42-): ≤100 mg/kg

cation traces

Ca: ≤5 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Na: ≤300 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Zn: ≤5 mg/kg

storage temp.

2-8°C

SMILES string

[H]C(=O)C([H])=O

InChI

1S/C2H2O2/c3-1-2-4/h1-2H

InChI key

LEQAOMBKQFMDFZ-UHFFFAOYSA-N

General description

Glyoxal is a nucleic acid denaturant. It acts as a specific probe for homopurine strands of DNA.

Application

Suitable for
  • nucleic acid denaturation
  • binding of DNA/RNA to nitrocellulose membrane

Biochem/physiol Actions

Glyoxal forms stable complex with guanine residues that results in denaturation of nucleic acids.

Other Notes

May precipitate on storage; redissolve at 50-60 °C.
This form of glyoxal is composed of 3 moles of glyoxal and 2 moles of water in a relatively stable configuration.

Disclaimer

May precipitate on storage; redissolve at 50-60 °C.


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pictograms

Health hazardExclamation mark

signalword

Warning

Hazard Classifications

Acute Tox. 4 Inhalation - Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter



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Hybridization of denatured RNA transferred or dotted nitrocellulose paper.
P S Thomas
Methods in enzymology, 100, 255-266 (1983-01-01)
R N Skopp et al.
Analytical biochemistry, 169(1), 132-137 (1988-02-15)
This paper describes agarose gel electrophoresis and silver staining of denatured RNAs. Glyoxal- or formaldehyde-denatured RNAs are electrophoresed in an agarose gel cast on a plastic support using an inert, low conductivity buffer. Following electrophoresis, the gel is stained with
M Vojtisková et al.
Journal of biomolecular structure & dynamics, 5(2), 283-296 (1987-10-01)
Plasmid pEJ4, which is a derivative of pUC19 containing an insert with 60-bp-long homopurine.homopyrimidine tract from sea urchin P. miliaris histone gene spacer, was studied by chemical probes of the DNA structure osmium tetroxide and glyoxal. The former probe reacts



Global Trade Item Number

SKUGTIN
50649-25ML04061832449722
50649-100ML04061838177032