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Merck
CN

50981

Guanidine thiocyanate

BioReagent, Molecular Biology, ≥99%

Synonym(s):

Guanidinium rhodanide, Guanidinium thiocyanate

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About This Item

Linear Formula:
NH2C(=NH)NH2 · HSCN
CAS Number:
593-84-0
Molecular Weight:
118.16
UNSPSC Code:
12352107
NACRES:
NA.52
PubChem Substance ID:
57651291
EC Number:
209-812-1
Beilstein/REAXYS Number:
3563461
MDL number:
MFCD00013027

Key Documents

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Product Name

Guanidine thiocyanate, BioReagent, Molecular Biology, ≥99%

InChI key

ZJYYHGLJYGJLLN-UHFFFAOYSA-N

InChI

1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H

SMILES string

SC#N.NC(N)=N

biological source

synthetic

grade

Molecular Biology

product line

BioReagent

assay

≥99%

form

powder

technique(s)

RNA extraction: suitable

impurities

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

color

colorless to white,

mp

115-122 °C
120-122 °C (lit.)

solubility

H2O: 4 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤5000 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤5000 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

storage temp.

room temp

Quality Level

200

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Application

Chaotropic agent and strong denaturant; solubilizes cells.
Guanidine thiocyanate has been used as a component of the stabilization buffer for RNA lysis. It can be used for deactivation of endogenous RNases leading to denaturation of nucleoprotein complexes. It is also suitable for denaturation of macromolecules such as nucleic acids and proteins and for RNA isolation.

Biochem/physiol Actions

Guanidine thiocyanate (GTC) plays a role in protein denaturation. It is involved in separation of ribosomal ribonucleic acid (rRNA) from ribosomes and denatures ribonuclease (RNase). GTC has been studied in the unfolding and refolding of fluorescent proteins (FP). It can be used along with phenol and chloroform for extraction of RNA.
Guanidine thiocyanate interferes with the hydrogen bonds and intramolecular interactions of macromolecules. It solubilizes the proteins resulting in disintegration of cellular structures.

pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

supp_hazards

EUH032

Storage Class

8B - Non-combustible corrosive hazardous materials

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
WXBF7771V
WXBF7615V
WXBF7646V
WXBF7366V
WXBF6939V

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Siun Chee Tan et al.
Journal of biomedicine & biotechnology, 2009, 574398-574398 (2009-12-17)
Extraction of DNA, RNA, and protein is the basic method used in molecular biology. These biomolecules can be isolated from any biological material for subsequent downstream processes, analytical, or preparative purposes. In the past, the process of extraction and purification
Standard methods for molecular research in Apis mellifera
Evans J D, et al.
Journal of Apicultural Research, 52, 1-54 (2013)
Nasir Ali et al.
BioMed research international, 2017, 9306564-9306564 (2017-08-09)
Nucleic acid extraction (NAE) plays a vital role in molecular biology as the primary step for many downstream applications. Many modifications have been introduced to the original 1869 method. Modern processes are categorized into chemical or mechanical, each with peculiarities
Isolation and Purification of RNA
Surzycki S
Basic Techniques in Molecular Biology, 119-144 (2000)
Extraction and Purification of Nucleic Acid using CBNP and PCIA Technique
Soni N
PharmaTutor, 4, 20-22 (2016)
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