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Merck
CN

51456

Urea

BioUltra, Molecular Biology, 99% (T)

Synonym(s):

Carbamide, Carbonyldiamide

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About This Item

Linear Formula:
NH2CONH2
CAS Number:
Molecular Weight:
60.06
UNSPSC Code:
12352111
NACRES:
NA.25
PubChem Substance ID:
EC Number:
200-315-5
Beilstein/REAXYS Number:
635724
MDL number:
Assay:
99% (T)
Grade:
Molecular Biology
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grade

Molecular Biology

Quality Level

product line

BioUltra

assay

99% (T)

form

powder or crystals

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.1% biuret, <0.005% formaldehyde (HCHO)

ign. residue

≤0.01% (as SO4)

loss

≤0.1% loss on drying, 20 °C (HV)

pH

8.0-10.0 (20 °C, 8 M in H2O)

mp

132-135 °C (lit.)

solubility

H2O: 8 M at 20 °C, clear, colorless

density

1.335 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤5 mg/kg, sulfate (SO42-): ≤10 mg/kg

SMILES string

NC(N)=O

cation traces

Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

λ

8 M in H2O

UV absorption

λ: 260 nm Amax: ≤0.03, λ: 280 nm Amax: ≤0.02

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

Gene Information

human ... CA1(759), CA2(760)
rat ... Ppm1a(24666)

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Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Other Notes

Denaturing reagent; Commonly used in conjunction with polyacrylamide gel electrophoresis for the fractionation of single-stranded nucleic acids with chains of up to 200 nucleotides

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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R. Poulson et al.
The Ribonucleic Acids, 333-333 (1977)
Fractionation of low molecular weight DNA or RNA in polyacrylamide gels containing 98% formamide or 7 M urea.
T Maniatis et al.
Methods in enzymology, 65(1), 299-305 (1980-01-01)
A Paulus et al.
Electrophoresis, 14(1-2), 27-35 (1993-01-01)
DNA digest analysis with polymer-filled capillaries in capillary electrophoresis is described. The samples analyzed consisted of commercially available standards including a 100 base pair ladder with repeating units up to 2000 base pairs. Three DNA digests covered the most common
R.C. Ogden et al.
Methods in Enzymology, 152, 66-66 (1987)
A mechanically and electrically self-healing supercapacitor.
Hua Wang et al.
Advanced materials (Deerfield Beach, Fla.), 26(22), 3638-3643 (2014-02-21)

Protocols

An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).

基于优化的 LC-MS/MS 工作流程,通过过滤辅助样品制备 (FASP) 对阿达木单抗 (Humira) 绘制低伪影胰蛋白酶酶解和肽图谱。

Global Trade Item Number

SKUGTIN
M5750-1KG04061826293386
M5750-25G04061834060307
M2518-100G04061835568819
M5750-100G04061834060291
V900396-5G04061832094236
Y000177904061833828656
H7125-25G04061832091273
3779-1GM07790788049744
3779-5GM04055977213027
H7125-1G04061833803295
H7125-5G04061833803318
V900396-1G04061825983868
59964-100MG04061832644769
51456-2.5KG04061832492988
51456-500G04061832492995

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