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Sigma-Aldrich

Phalloidin–Atto 594

suitable for fluorescence, ≥90.0% (HPLC)

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Synonym(s):
Atto 594–Phalloidin
NACRES:
NA.32

Quality Level

100

Assay

≥90.0% (HPLC)

form

solid

manufacturer/tradename

ATTO-TEC GmbH

λ

in acetonitrile: water (3:7)

UV absorption

λ: 600-606 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

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General description

Atto 594 is a novel fluorescent label belonging to the class of Rhodamine dyes. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high thermal and photo-stability, excellent water solubility, and very little triplet formation. After coupling to a substrate Atto 594 carries a net electrical charge of -1.
Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind F actin in liver and muscle cells. As a result of binding phalloidin, actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin, and not to monomeric actin. The dissociation constant of the actin-phalloidin complex has been determined to be on the order of 3 x 10-8. Phalloidin differs from amanitin in rapidity of action; at high dose levels, death of mice or rats occurs within 1 or 2 hours. Fluorescent conjugates of phalloidin are used to label actin filaments for histological applications. Some structural features of phalloidin are required for the binding to actin. However, the side chain of amino acid 7 (g-d-dihydroxyleucine) is accessible for chemical modifications without appreciable loss of affinity for actin.

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Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Regulatory Information

高风险级别生物产品--毒素类产品

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Anika M Helferich et al.
Cellular and molecular life sciences : CMLS, 75(23), 4301-4319 (2018-07-22)
Genetic and functional studies suggest diverse pathways being affected in the neurodegenerative disease amyotrophic lateral sclerosis (ALS), while knowledge about converging disease mechanisms is rare. We detected a downregulation of microRNA-1825 in CNS and extra-CNS system organs of both sporadic
Therese B Deramaudt et al.
International journal of molecular medicine, 45(6), 1927-1941 (2020-04-24)
Macrophages are active contributors to the innate immune defense system. As macrophage activation is clearly affected by the surrounding microenvironment, the present study investigated the effect of sulforaphane (SFN) on the bactericidal activity of macrophages and the underlying molecular mechanisms involved

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