Anti-Rabbit IgG−Abberior^® STAR 635P antibody produced in goat

Abberior STAR 635P was the first member of an entirely new class of fluorescent dyes introduced exclusively by Abberior: the class of phosphorylated fluorescent dye. The dye excels in its photophysical parameters: it is very bright, very stable and it enables basically background free imaging. The dye works exceptionally well with Abberior Instruments STEDYCON und Expert line microscopes and Leica STED microscopes. Abberior STAR 635P can substitute Atto^™ 647N, Alexa Fluor^™ 647, or Cy^®5. It can be excited with lasers at wavelength between 630 - 650 nm. For STED, a depletion wavelength around 750 - 800 nm is recommended. Abberior STAR 635P is the ideal partner for Abberior STAR 580 to obtain optimal 2 Color STED results. Best results are obtained with freshly prepared samples.

Photophysical properties (carboxylic acid):
Absorption Maximum, λ_ex [nm]: 638 (PBS pH 7.4), 638 (H₂O), 634 (MeOH)
Extinction Coefficient, ε_max [M^-1cm^-1]: 120 000 (PBS pH 7.4), 120 000 (H₂O), 130 000 (MeOH)
Correction Factor, CF₂₆₀ = ε₂₆₀/ε_max: 0,21 (PBS pH 7.4)
Correction Factor, CF₂₈₀ = ε₂₈₀/ε_max: 0,40 (PBS pH 7.4)
Fluorescence Maximum, λ_em [nm]: 651 (PBS pH 7.4), 651 (H₂O), 651 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,90 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,3 (PBS pH 7.4)

Designed and tested for fluorescent super-resolution microscopy

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

ALEXA FLUOR is a trademark of Life Technologies

Atto is a trademark of Atto-Tec GmbH

Cy is a registered trademark of Cytiva

abberior is a registered trademark of Abberior GmbH