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53608

Biotin (5-fluorescein) conjugate

Name: Biotin (5-fluorescein) conjugate
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥90% (HPCE)

Synonym(s):

N-(Biotinylamidoethyl)-fluorescein-5-carboxamide, N-(Fluorescein-5-carbamidoethyl)biotinamide

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About This Item

Empirical Formula (Hill Notation):

C₃₃H₃₂N₄O₈S

CAS Number:

957494-27-8

Molecular Weight:

644.69

UNSPSC Code:

12352108

eCl@ss:

34058011

PubChem Substance ID:

57651424

NACRES:

NA.32

MDL number:

MFCD03791084

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Properties

product line

BioReagent

Quality Level

100

assay

≥90% (HPCE)

solubility

DMSO: soluble

fluorescence

λ_ex 490 nm; λ_em 524 nm in 0.1 M Tris pH 8.0

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

[H][C@]12CS[C@@H](CCCCC(=O)NCCNC(=O)c3ccc(c(c3)C(O)=O)C4=C5C=CC(=O)C=C5Oc6cc(O)ccc46)[C@@]1([H])NC(=O)N2

InChI

1S/C33H32N4O8S/c38-18-6-9-21-25(14-18)45-26-15-19(39)7-10-22(26)29(21)20-8-5-17(13-23(20)32(42)43)31(41)35-12-11-34-28(40)4-2-1-3-27-30-24(16-46-27)36-33(44)37-30/h5-10,13-15,24,27,30,38H,1-4,11-12,16H2,(H,34,40)(H,35,41)(H,42,43)(H2,36,37,44)/t24-,27-,30-/m0/s1

InChI key

DFUFXKZUEOKPSD-LFERIPGTSA-N

Description

Application

Biotin (5-fluorescein) conjugate is a reagent that may be used in situations similar to fluorescein isothiocyanate staining to label avidin/streptavidin conjugated molecules such as antibodies with the fluorophore 5-fluorescein.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Biotin-fluorescein conjugate for the fluorometric, accurate measurement of avidin and streptavidin.

Often referred to as Biotin (4-fluorescein) conjugate.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Global Trade Item Number

SKU	GTIN
53608-5MG-F	04061832532509
53608-1MG-F	04061823354530