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Merck
CN

54702

FITC–DEAE–Dextran

average mol wt 70,000

Synonym(s):

FITC–DEAE–Dextran 70, Fluorescein isothiocyanate–Diethylaminoethyl–Dextran

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352108

Key Documents

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Product Name

FITC–DEAE–Dextran, average mol wt 70,000

biological source

synthetic

conjugate

FITC conjugate

form

powder or crystals

mol wt

average mol wt 70,000

composition

nitrogen content, 2-5%

extent of labeling

0.001-0.020 mol/mol FITC glucose

technique(s)

permeability assay: suitable

fluorescence

λex 493 nm; λem 518 nm in 0.1 M Tris pH 8.0

Quality Level

100

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Application

FITC-DEAE-Dextran 70 is a cationic fluorescent dextran that may be used to study cell processes such as cell permeability, phagocytosis and endocytosis and to study mechanisms of biomolecular delivery. FITC-DEAE-Dextran 70 may be use to compare its selectivity versus neutral FITC-Dextran 70 or anionic FITC-CM-Dextran 70.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN9763
BCCK8613
BCCG9465
BCCD0843
BCBS2902V

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J K Leypoldt et al.
Kidney international, 43(4), 837-844 (1993-04-01)
The influence of molecular charge on macromolecule transport during peritoneal dialysis was assessed by determining transperitoneal transport rates for fluorescent-labeled macromolecules (molecular radii from 15 to 40 A) that differed only in molecular charge: neutral dextran, anionic dextran sulfate and
Mary C Regier et al.
Scientific reports, 9(1), 6433-6433 (2019-04-25)
Concentration gradients of biochemical stimuli such as morphogens play a critical role in directing cell fate patterning across species and throughout development but are not commonly recapitulated in vitro. While in vitro biomolecule gradients have been generated using customized microfluidic
Carrier and dose effects on the pharmacokinetics of T-0128, a camptothecin analogue-carboxymethyl dextran conjugate, in non-tumor- and tumor-bearing rats.
Harada M, Murata J, Sakamura Y, et al.
J. Controlled Release, 71, 71-86 (2001)
Takayuki Morikawa et al.
Scientific reports, 10(1), 6422-6422 (2020-04-15)
Physiological regulation of blood flow in bone marrow is important to maintain oxygen and glucose supplies but also the physiological hypoxic state of the hematopoietic stem cell (HSC) niche. However, regulatory mechanisms underlying microcirculation in the bone marrow (BM) niche
Reduced diffusion of charge-modified, conformationally intact anionic Ficoll relative to neutral Ficoll across the rat glomerular filtration barrier in vivo.
Axelsson J, Sverrisson K, Rippe A, et al.
American Journal of Physiology: Renal Physiology, 301, F708-F712 (2011)

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