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56499C

Dulbecco’s Modified Eagle’s Medium/High Modified

with 4500 mg/L dextrose, with 4.0 mM L-glutamine, with 110 mg/L sodium pyruvate, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

Dulbecco’s Modified Eagle’s Medium - high glucose, DME, DMEM

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About This Item

NACRES:
NA.71
UNSPSC Code:
12352200
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Product Name

Dulbecco′s Modified Eagle′s Medium/High Modified, with 4500 mg/L dextrose, with 4.0 mM L-glutamine, with 110 mg/L sodium pyruvate, without sodium bicarbonate, dry powder, suitable for cell culture DME, DMEM, Dulbecco’s Modified Eagle’s Medium – high Glucose. Use 13.49 g/L See information sheet, 56499C @ www.sigmaaldrich.com for hydration instructions.

description

for research or for further manufacturing use

Quality Segment

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

phenol red: yes
sodium pyruvate: yes
L-glutamine: yes
glucose: high

shipped in

ambient

storage temp.

2-8°C

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Preparation Note

Formulated to contain 13.5 grams of powder per liter of medium.

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Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

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