56575
GenElute™-LPA
Neutral carrier for ethanol precipitation
Synonym(s):
linear polyacrylamide
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About This Item
NACRES:
NA.52
UNSPSC Code:
12352200
Product Name
GenElute™-LPA, Neutral carrier for ethanol precipitation
storage temp.
2-8°C
Quality Level
Application
GenElute™-LPA has been used to:
- extract DNA from cyanobacterial and algal cultures
- precipitate genomic DNA
- extract and purify DNA from snap-frozen uveal melanoma tissues
- to extract RNA from isolated CD4+ cells
Biochem/physiol Actions
GenElute LPA Linear Polyacrylamide is an efficient neutral carrier for precipitating picogram amounts of nucleic acids with ethanol. The nucleic acid precipitate can be collected simply by centrifugation. LPA offers several advantages for recovering DNA or studying DNA-protein interactions, relative to other carriers, such as tRNA or glycogen. tRNA interferes with DNA during phosphorylation with polynucleotide kinase and glycogen competes with protein in DNA-protein interaction studies. In contrast, LPA is completely inert. LPA is synthesized chemically and, therefore, is not contaminated with biological material. The precipitate is visible immediately upon addition of LPA, thus eliminating wait time and low temperature incubation.
The presence of LPA during ethanol precipitation results in complete recovery of fragments larger than 20 base pairs, whereas most of the DNA is lost if no carrier is used. Very short DNA fragments, less than 20 base pairs, do not coprecipitate with LPA, allowing separation of labeled DNA from unreacted nucleotides by precipitation after the labeling reaction.
Packaging
GenElute LPA is tested for nucleases, and is supplied in nuclease-free water as a 25 mg/ml solution.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
General description
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Quantitative PCR enumeration of total/toxic Planktothrix rubescens and total cyanobacteria in preserved DNA isolated from lake sediments.
Savichtcheva O
Applied and Environmental Microbiology, 77(24), 8744-8753 (2011)
Peripheral immune responses in pregnant cattle following Neospora caninum infection.
Rosbottom A
Parasite Immunology, 29(4), 219-228 (2007)
Ethanol precipitation of DNA with linear polyacrylamide as carrier.
C Gaillard et al.
Nucleic acids research, 18(2), 378-378 (1990-01-25)
Promoter de-methylation of cyclin D2 by sulforaphane in prostate cancer cells.
Hsu A
Clinical epigenetics, 3, 3-3 (2011)
Whole-genome microarray detects deletions and loss of heterozygosity of chromosome 3 occurring exclusively in metastasizing uveal melanoma.
Lake SL
Investigative Ophthalmology & Visual Science, 51(10), 4884-4891 (2010)
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56575 GenElute LPA
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