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56759

Atto 425

Name: Atto 425
Brand: SIGMA

BioReagent, suitable for fluorescence

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About This Item

Empirical Formula (Hill Notation):

C₂₂H₂₇NO₆

CAS Number:

652966-03-5

Molecular Weight:

401.45

MDL number:

MFCD32670704

UNSPSC Code:

12352108

PubChem Substance ID:

329758237

NACRES:

NA.32

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product line

BioReagent

Quality Level

100

assay

≥90% (HPLC)

form

(Report result)

manufacturer/tradename

ATTO-TEC GmbH

transmittance

254 nm, >90%, 425 nm, >90%

fluorescence

λ_ex 440 nm; λ_em 484 nm

λ

in PBS, pH 7.4, in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 423-429 nm A_max

suitability

suitable for fluorescence

shipped in

wet ice

storage temp.

−20°C

SMILES string

CCOC(=O)C1=Cc2cc3C(C)CC(C)(C)N(CCCC(O)=O)c3cc2OC1=O

InChI

1S/C22H27NO6/c1-5-28-20(26)16-10-14-9-15-13(2)12-22(3,4)23(8-6-7-19(24)25)17(15)11-18(14)29-21(16)27/h9-11,13H,5-8,12H2,1-4H3,(H,24,25)

InChI key

WNDDWSAHNYBXKY-UHFFFAOYSA-N

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 425 is suitable for preparation of fluorescence labeling reagents.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Frequency domain fluorescence microspectrometry: Application to cellular uptake and drug distribution.

Praus, P., et al.

Spectroscopy: An International Journal, 24, 303-307 (2010)

Interaction of thioflavin T with amyloid fibrils: stoichiometry and affinity of dye binding, absorption spectra of bound dye.

Anna I Sulatskaya et al.

The journal of physical chemistry. B, 115(39), 11519-11524 (2011-08-26)

The fluorescence of the benzothiazole dye thioflavin T (ThT) is a well-known test for amyloid fibril formation. It has now become evident that ThT can also be used for structural investigations of amyloid fibrils and even for the treatment of

Asymmetric printing of molecules and zeolites on self assembled monolayers.

Nermin Seda Kehr et al.

Nanoscale, 2(4), 601-605 (2010-07-21)

Microcontact printing (mCP) is used to immobilize dyes and peptides asymmetrically, by a "peptide coupling" reaction, on monolayers of zeolite L crystals in the contact area between the stamp and the surface of the monolayer. Chemically patterned surfaces of monolayers

Suborganelle sensing of mitochondrial cAMP-dependent protein kinase activity.

Richard S Agnes et al.

Journal of the American Chemical Society, 132(17), 6075-6080 (2010-04-13)

A fluorescent sensor of protein kinase activity has been developed and used to characterize the compartmentalized location of cAMP-dependent protein kinase activity in mitochondria. The sensor functions via a phosphorylation-induced release of a quencher from a peptide-based substrate, producing a

Triple-color coincidence analysis: one step further in following higher order molecular complex formation.

Katrin G Heinze et al.

Biophysical journal, 86(1 Pt 1), 506-516 (2003-12-26)

Confocal fluorescence spectroscopy is a versatile method for studying dynamics and interactions of biomolecules in their native environment with minimal interference with the observed system. Analyzing coincident fluctuations induced by single molecule movement in spectrally distinct detection channels, dual-color fluorescence

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Global Trade Item Number

SKU	GTIN
V4877-5MG	04061832943503
V4877-1MG	04061837481062
56759-1MG-F	04061826687918

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