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Merck
CN

57000

Indigo carmine

suitable for microscopy

Synonym(s):

Acid Blue 74, Indigo-5,5′-disulfonic acid disodium salt, Indigocarmine

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About This Item

Empirical Formula (Hill Notation):
C16H8N2Na2O8S2
CAS Number:
Molecular Weight:
466.35
EC Number:
212-728-8
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
MDL number:
Colour Index Number:
73015
Beilstein/REAXYS Number:
4103904
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form

powder

Quality Level

technique(s)

microbe id | staining: suitable

loss

≤5% loss on drying

pH

3.87 (30 °C, 7.14 g/L)

mp

>340 °C

solubility

methanol: 0.01 g/10 mL, blue to very deep blue

density

0.71 g/cm3 at 29 °C

εmax

≥350 at 603-613 nm

suitability

suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[O-]S(=O)(=O)c1ccc2NC(\C(=O)c2c1)=C3\Nc4ccc(cc4C3=O)S([O-])(=O)=O

InChI

1S/C16H10N2O8S2.2Na/c19-15-9-5-7(27(21,22)23)1-3-11(9)17-13(15)14-16(20)10-6-8(28(24,25)26)2-4-12(10)18-14;;/h1-6,17-18H,(H,21,22,23)(H,24,25,26);;/q;2*+1/p-2/b14-13+;;

InChI key

KHLVKKOJDHCJMG-QDBORUFSSA-L

Application

Indigo carmine has been used in agarose plates to study behavioral feeding assays in Drosophila larvae. It has also been used for macroscopic assessment of intestinal adenomatous polyps isolated from mice.

Biochem/physiol Actions

Indigo carmine is a contrast dye which helps in highlighting mucosal changes. It accumulates in crevices, cysts and foveolae and stains the tissue blue. The abnormal tissue remains translucent pink.


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pictograms

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signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

ppe

dust mask type N95 (US), Eyeshields, Gloves



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Endoscopic Imaging Techniques and Tools (2006)
CDC42 inhibition suppresses progression of incipient intestinal tumors.
Sakamori R, et al.
Cancer Research, 74, 5480-5480 (2014)
A Pair of Pharyngeal Gustatory Receptor Neurons Regulates Caffeine-Dependent Ingestion in Drosophila Larvae.
Choi J, et al.
Frontiers in Cellular Neuroscience, 10, 181-181 (2016)



Global Trade Item Number

SKUGTIN
57000-500G-F04061832597317
57000-100G-F04061832597300
57000-25G-F04061835521005