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Merck
CN

57000

Indigo carmine

suitable for microscopy

Synonym(s):

Acid Blue 74, Indigo-5,5′-disulfonic acid disodium salt, Indigocarmine

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About This Item

Empirical Formula (Hill Notation):
C16H8N2Na2O8S2
CAS Number:
860-22-0
Molecular Weight:
466.35
EC Number:
212-728-8
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
57651616
MDL number:
MFCD00005723
Colour Index Number:
73015
Beilstein/REAXYS Number:
4103904

Key Documents

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InChI key

KHLVKKOJDHCJMG-QDBORUFSSA-L

InChI

1S/C16H10N2O8S2.2Na/c19-15-9-5-7(27(21,22)23)1-3-11(9)17-13(15)14-16(20)10-6-8(28(24,25)26)2-4-12(10)18-14;;/h1-6,17-18H,(H,21,22,23)(H,24,25,26);;/q;2*+1/p-2/b14-13+;;

SMILES string

[Na+].[Na+].[O-]S(=O)(=O)c1ccc2NC(\C(=O)c2c1)=C3\Nc4ccc(cc4C3=O)S([O-])(=O)=O

form

powder

technique(s)

microbe id | staining: suitable

loss

≤5% loss on drying

pH

3.87 (30 °C, 7.14 g/L)

mp

>340 °C

solubility

methanol: 0.01 g/10 mL, blue to very deep blue

density

0.71 g/cm3 at 29 °C

εmax

≥350 at 603-613 nm

suitability

suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

100

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Application

Indigo carmine has been used in agarose plates to study behavioral feeding assays in Drosophila larvae. It has also been used for macroscopic assessment of intestinal adenomatous polyps isolated from mice.

Biochem/physiol Actions

Indigo carmine is a contrast dye which helps in highlighting mucosal changes. It accumulates in crevices, cysts and foveolae and stains the tissue blue. The abnormal tissue remains translucent pink.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN8254
BCCL8111
BCCF3080
BCCF3079
BCCC4716

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Choi J, et al.
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Aurora rings: a novel endoscopic finding to distinguish inverted colonic diverticula from colon polyps.
Michael D Share et al.
Gastrointestinal endoscopy, 77(2), 308-312 (2012-12-05)
Three-dimensional staining of liver segments with an ultrasound contrast agent as an aid to anatomic liver resection.
Junichi Shindoh et al.
Journal of the American College of Surgeons, 215(2), e5-10 (2012-06-12)
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