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Merck
CN

57000

Indigo carmine

suitable for microscopy

Synonym(s):

Acid Blue 74, Indigo-5,5′-disulfonic acid disodium salt, Indigocarmine

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About This Item

Empirical Formula (Hill Notation):
C16H8N2Na2O8S2
CAS Number:
860-22-0
Molecular Weight:
466.35
EC Number:
212-728-8
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
57651616
MDL number:
MFCD00005723
Colour Index Number:
73015
Beilstein/REAXYS Number:
4103904

Key Documents

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Product Name

Indigo carmine, suitable for microscopy

InChI key

KHLVKKOJDHCJMG-QDBORUFSSA-L

InChI

1S/C16H10N2O8S2.2Na/c19-15-9-5-7(27(21,22)23)1-3-11(9)17-13(15)14-16(20)10-6-8(28(24,25)26)2-4-12(10)18-14;;/h1-6,17-18H,(H,21,22,23)(H,24,25,26);;/q;2*+1/p-2/b14-13+;;

SMILES string

[Na+].[Na+].[O-]S(=O)(=O)c1ccc2NC(\C(=O)c2c1)=C3\Nc4ccc(cc4C3=O)S([O-])(=O)=O

form

powder

technique(s)

microbe id | staining: suitable

loss

≤5% loss on drying

pH

3.87 (30 °C, 7.14 g/L)

mp

>340 °C

solubility

methanol: 0.01 g/10 mL, blue to very deep blue

density

0.71 g/cm3 at 29 °C

εmax

≥350 at 603-613 nm

suitability

suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

100

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Application

Indigo carmine has been used in agarose plates to study behavioral feeding assays in Drosophila larvae. It has also been used for macroscopic assessment of intestinal adenomatous polyps isolated from mice.

Biochem/physiol Actions

Indigo carmine is a contrast dye which helps in highlighting mucosal changes. It accumulates in crevices, cysts and foveolae and stains the tissue blue. The abnormal tissue remains translucent pink.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN8254
BCCL8111
BCCF3080
BCCF3079
BCCC4716

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CDC42 inhibition suppresses progression of incipient intestinal tumors.
Sakamori R, et al.
Cancer Research, 74, 5480-5480 (2014)
Endoscopic Imaging Techniques and Tools (2006)
A Pair of Pharyngeal Gustatory Receptor Neurons Regulates Caffeine-Dependent Ingestion in Drosophila Larvae.
Choi J, et al.
Frontiers in Cellular Neuroscience, 10, 181-181 (2016)
Tsuneo Oyama
Digestive endoscopy : official journal of the Japan Gastroenterological Endoscopy Society, 25 Suppl 1, 7-12 (2013-03-27)
The indication of endoscopic submucosal dissection for Barrett's esophageal adenocarcinoma (BEA) is superficial BEA without lymph node metastasis. The characteristic endoscopic findings of superficial BEA are elevation, depression, and color change. Indigocarmine spreading is useful for the diagnosis of lateral
Christian Gómez-Solís et al.
Journal of hazardous materials, 217-218, 194-199 (2012-04-03)
Indigo carmine and methylene blue dyes in aqueous solution were photodegraded using SiC-TiO(2) catalysts prepared by sol-gel method. After thermal treatment at 450°C, SiC-TiO(2) catalysts prepared in this work showed the presence of SiC and TiO(2) anatase phase. Those compounds
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