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COO/COA

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57180

Indo 1

Name: Indo 1
Brand: SIGMA

suitable for fluorescence, ≥80.0% (HPLC)

Synonym(s):

4-(6-Carboxy-2-indolyl)-4′-methyl-2,2′-(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid pentapotassium salt

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About This Item

Empirical Formula (Hill Notation):

C₃₂H₂₆K₅N₃O₁₂

CAS Number:

132319-56-3

Molecular Weight:

840.05

MDL number:

MFCD00077797

UNSPSC Code:

12352200

PubChem Substance ID:

329758276

NACRES:

NA.32

Assay

≥80.0% (HPLC)

form

solid

fluorescence

λ_ex 331 nm; λ_em 398 nm in 0.1 M Tris pH 8.0, 10 mM Ca²⁺
λ_ex 349 nm; λ_em 482 nm (100mM KCI)

suitability

in accordance for fluorescence
suitable for fluorescence

storage temp.

−20°C

SMILES string

[K+].[K+].[K+].[K+].[K+].Cc1ccc(N(CC([O-])=O)CC([O-])=O)c(OCCOc2cc(ccc2N(CC([O-])=O)CC([O-])=O)-c3cc4ccc(cc4[nH]3)C([O-])=O)c1

InChI

1S/C32H31N3O12.5K/c1-18-2-6-24(34(14-28(36)37)15-29(38)39)26(10-18)46-8-9-47-27-13-20(5-7-25(27)35(16-30(40)41)17-31(42)43)22-11-19-3-4-21(32(44)45)12-23(19)33-22;;;;;/h2-7,10-13,33H,8-9,14-17H2,1H3,(H,36,37)(H,38,39)(H,40,41)(H,42,43)(H,44,45);;;;;/q;5*+1/p-5

InChI key

VGZPRMSXVNDMAN-UHFFFAOYSA-I

Application

Indo 1, a fluorescent calcium chelator and indicator, is used to monitor cytoplasmic Ca2+ transients induced by various physiological and mechanical processes.

Other Notes

Fluorescent calcium chelator and indicator with an unusually large shift in fluorescence emission from 480 nm to 400 nm on binding of calcium. Useful for intracellular application

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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