61911
Atto 520 azide
suitable for fluorescence
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About This Item
UNSPSC Code:
12352200
assay
≥80.0% (HPCE)
form
solid
mol wt
Mw 681 g/mol
manufacturer/tradename
ATTO-TEC GmbH
fluorescence
λex 516 nm; λem 545 nm±10 nm in PBS, pH 7.4
suitability
suitable for fluorescence
storage temp.
−20°C
General description
Atto 520 is a novel fluorescent label related to the well-known dye Rhodamine 6G. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. The dye is moderately hydrophilic. Above pH 7.0 the dye exists in an equilibrium with a colourless form (pseudobase). This is a reversible reaction, which does not interfere with the process of coupling to proteins etc. On lowering the pH absorption and fluorescence are restored fully. For details of coupling see our recommended labeling procedures The fluorescence is excited most efficiently in the range 510 - 535 nm. A suitable source of excitation is an Argon-Ion laser using the 514 nm line.
The azide modification is suitable for reactions with alkyne groups (Huisgen reaction - “Click Chemistry“).
find more information here
The azide modification is suitable for reactions with alkyne groups (Huisgen reaction - “Click Chemistry“).
find more information here
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Complex dynamics of combined long-range fluorescence resonance energy transfer (FRET) and short-range electron transfer (ET) processes in a single double-stranded DNA (dsDNA) molecule (see figure) reveals that FRET remains almost unaltered in the presence of ET. Present systems also demonstrate