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62310

Sigma-Aldrich

Lipase from Rhizopus niveus

powder (fine), ≥1.5 U/mg

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Synonym(s):
Triacylglycerol acylhydrolase, Triacylglycerol lipase
CAS Number:
9001-62-1
Enzyme Commission number:
3.1.1.3 (BRENDA, IUBMB)
EC Number:
232-619-9
MDL number:
MFCD00131509
NACRES:
NA.26

biological source

fungus (Rhizopus niveus)

Quality Level

100

form

powder (fine)

specific activity

≥1.5 U/mg

mol wt

Mr ~83000

storage temp.

2-8°C

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

InChI key

QWZUIMCIEOCSJF-CHHCPSLASA-N

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General description

Lipases hydrolyze ester bonds of triacylglycerols and in some conditions, catalyze the synthesis of ester bonds by transesterification. Lipase from Rhizopus niveus exists as a single polypeptide chain with a molecular mass of about 30-34kDa.

Application

Lipase from Rhizopus niveus has been used to determine the positional distribution of fatty acids within triacylglycerol by hydrolysis of the acyl ester linkage at C-1 and C-3. It has also been used to induce intragastric lipolysis and study its effects on felodipine (a medication) release from a hydrophilic, extended release tablet.

Biochem/physiol Actions

Lipase from Rhizopus niveus is specifically used to produce cocoa butter substitutes.
Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 μmol fatty acid from a triglyceride per minute at pH 7.7 and 40°C (olive oil as substrate)]; 300 U as described above are equivalent to ~1 U using triolein, Cat. No. 62314, at pH 8.0 and 40°C as substrate.

Other Notes

Note: When triacetin is used as substrate, the pH is 7.4. Incubation time: 60 minutes.
Substrate specificity: preferentially hydrolyzes the 1- and 3-positions(middle to long fatty acids); optimum pH 5.0-7.0; optimum temperature 30-45°C; Selective enzymatic removal of protecting functions (C-terminal COOH function)

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Analysis of triacylglycerol accumulation under nitrogen deprivation in the red alga Cyanidioschyzon merolae
Masakazu Toyoshima
Microbiology (2016)
Y. H. Hui and George G. Khachatourians
Food Biotechnology (1995)
P. Braun et al.
Synlett, 105-105 (1990)
Simulation of gastric lipolysis and prediction of felodipine release from a matrix tablet in the fed stomach
A.Diakidou
European Journal of Pharmaceutical Sciences (2009)
Purification, characterization and crystallization of two types of lipase from Rhizopus niveus
Mitsutaka Kohno
Bioscience, Biotechnology, and Biochemistry, 58(6) (1994)
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