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64958

2-Methoxy-2,4-diphenyl-3(2H)-furanone

Name: 2-Methoxy-2,4-diphenyl-3(2<I>H</I>)-furanone
Brand: SIGMA

suitable for fluorescence, ≥98.0% (HPLC)

Synonym(s):

2,4-Diphenyl-2-methoxy-3(2H)-furanone, MDPF

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About This Item

Empirical Formula (Hill Notation):

C₁₇H₁₄O₃

CAS Number:

50632-57-0

Molecular Weight:

266.29

UNSPSC Code:

12352108

NACRES:

NA.32

PubChem Substance ID:

329760016

MDL number:

MFCD00036834

Beilstein/REAXYS Number:

1288529

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Quality Level

100

assay

≥98.0% (HPLC)

form

solid

fluorescence

λ_ex 384 nm; λ_em 472 nm in acetonitrile (after derivatization with hexylamine)

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

COC1(OC=C(C1=O)c2ccccc2)c3ccccc3

InChI

1S/C17H14O3/c1-19-17(14-10-6-3-7-11-14)16(18)15(12-20-17)13-8-4-2-5-9-13/h2-12H,1H3

InChI key

BLWINLJDTOJSRU-UHFFFAOYSA-N

Application

2-Methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) is used as a fluorescence reagent to derivatize primary and secondary amines on molecules such as proteins. MDPF derivatized molecules can be detected during analytical and separation procedures including chromatography, electrophoresis and zymography.

Other Notes

Reagent for the derivatization of primary and secondary amines for HPLC; highly fluorescent derivatives are formed; Pre-column derivatization of amines; Fluorescent labeling of proteins before SDS-PAGE

pictograms

GHS07

signalword

Warning

hcodes

H315,H319,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Macromolecular transport across endothelial monolayers.

R C Schaeffer et al.

International journal of microcirculation, clinical and experimental, 11(2), 181-201 (1992-05-11)

We studied the macromolecular size-selective transport characteristics of polycarbonate (PC) filters with defined pore radius (rp; 15,000 to 400 A) as well as 2,000 A rp PC filter-bovine pulmonary artery endothelial cell (EC) monolayer sandwich under zero hydrostatic pressure conditions

Endocytosis and exocytosis of protein in capillary endothelium.

S K Williams et al.

Journal of cellular physiology, 120(2), 157-162 (1984-08-01)

The transport of proteins across continuous capillary endothelium is believed to be mediated by micropinocytic vesicles which shuttle molecules between the lumenal and abluminal plasma membrane. We have studied the ability of capillary endothelial cells isolated from rat epididymal fat

Rapid fluorescent monitoring of total protein patterns on sodium dodecyl sulfate-polyacrylamide gels and western blots before immunodetection and sequencing.

F J Alba et al.

Electrophoresis, 19(14), 2407-2411 (1998-11-20)

The fluorogenic dye 2-methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) has been used for the detection of total protein patterns on polyvinylidene difluoride (PVDF) membranes. Fluorescent staining of protein bands on membranes with this covalent dye is completed in 20 min. Wet membranes are translucent

Fluorescent monitoring of proteins during sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting.

B W Falk et al.

Analytical biochemistry, 144(2), 537-541 (1985-02-01)

Fluorescent labeling of proteins was found to be a very sensitive and reliable alternative to conventional methods for monitoring proteins on Western blots. Proteins were labeled with 2-methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) before SDS-PAGE. After electrophoresis and subsequent electro-blotting the fluorescent-labeled proteins were

S. Stein

Pept. Neurobiol., 9-9 (1977)

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Global Trade Item Number

SKU	GTIN
64958-100MG	04061832731667
64958-25MG	04061833277997

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