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72485

Sigma-Aldrich

Nile Red

for microscopy

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Synonym(s):
Nile Blue A Oxazone
Empirical Formula (Hill Notation):
C20H18N2O2
CAS Number:
Molecular Weight:
318.37
Beilstein:
279110
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.32

grade

for microscopy

Quality Level

form

crystals

mp

203-205 °C (lit.)

λmax

553 nm

SMILES string

CCN(CC)c1ccc2N=C3C(Oc2c1)=CC(=O)c4ccccc34

InChI

1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3

InChI key

VOFUROIFQGPCGE-UHFFFAOYSA-N

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This Item
N301319123N5632
Nile Red for microscopy

Sigma-Aldrich

72485

Nile Red

-
Nile Red Technical grade

Sigma-Aldrich

N3013

Nile Red

-
Nile Red BioReagent, suitable for fluorescence, ≥97.0% (HPLC)

Sigma-Aldrich

19123

Nile Red

Essential+ Grade
Nile Blue A Dye content ≥75 %

Sigma-Aldrich

N5632

Nile Blue A

-
form

crystals

form

powder

form

solid

form

powder

mp

203-205 °C (lit.)

mp

203-205 °C (lit.)

mp

203-205 °C (lit.), 205-215 °C

mp

>300 °C (dec.) (lit.)

λmax

553 nm

λmax

553 nm

λmax

553 nm

λmax

-

General description

Nile Red is a phenoxazone dye. It is an important stain, which is used to detect intracellular lipid droplets and proteins. Nile Red detects lysosomes and lysosome-related organelles, like gut granules in C. elegans intestinal cells. It exhibits strong fluorescence enhancement and is used for staining SDS (sodium dodecyl sulphate) gels.

Application

Nile Red has been used:
  • as a fluorescent dye for staining fat by confocal scanning laser microscopy (CSLM)
  • to label nanoparticles (NPs)
  • for lipid analysis

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Influence of shear during enzymatic gelation of caseinate-water and caseinate-water-fat systems
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Journal of Food Engineering, 79(2), 706-717 (2007)
Curcumin/Turmeric as an Environment-friendly Stain for Proteins on Acrylamide Gels
Polyphenols in Human Health and Disease, 779-784 (2014)
Analysis of membrane-bound organelles
Fares H and van der Bliek AM
Methods in Cell Biology, 107, 239-263 (2012)
Labeling nanoparticles: Dye leakage and altered cellular uptake
Snipstad S, et al.
Cytometry. Part A : the Journal of the International Society For Analytical Cytology, 91(8), 760-766 (2017)
Importance of intrinsic properties of dense caseinate dispersions for structure formation
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Biomacromolecules, 8(11), 3540-3547 (2007)

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