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75259

Sigma-Aldrich

Tris(2-carboxyethyl)phosphine hydrochloride

BioUltra, ≥98% (NMR)

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Synonym(s):
TCEP
Empirical Formula (Hill Notation):
C9H15O6P · HCl
CAS Number:
Molecular Weight:
286.65
Beilstein:
3724376
MDL number:
PubChem Substance ID:
NACRES:
NA.25

product line

BioUltra

Quality Level

Assay

≥98% (NMR)

form

crystals

reaction suitability

reagent type: reductant

loss

≤1.0% loss on drying

pH

1.2-1.5

solubility

H2O: 1 M, clear, colorless (no residue)

anion traces

sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.05 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

0.1 M in H2O

UV absorption

λ: 260 nm Amax: 0.05
λ: 280 nm Amax: 0.03

storage temp.

2-8°C

SMILES string

Cl[H].OC(=O)CCP(CCC(O)=O)CCC(O)=O

InChI

1S/C9H15O6P.ClH/c10-7(11)1-4-16(5-2-8(12)13)6-3-9(14)15;/h1-6H2,(H,10,11)(H,12,13)(H,14,15);1H

InChI key

PBVAJRFEEOIAGW-UHFFFAOYSA-N

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This Item
C4706646547580560
TCEP, Hydrochloride A water-soluble and odorless disulfide-reducing agent that is more stable and effective than DTT.

Millipore

580560

TCEP, Hydrochloride

-
assay

≥98% (NMR)

assay

-

assay

-

assay

≥98% (elemental analysis)

form

crystals

form

powder

form

-

form

crystalline solid

pH

1.2-1.5

pH

-

pH

7.0(aqueous solution; pH was adjusted with ammonium hydroxide)

pH

-

solubility

H2O: 1 M, clear, colorless (no residue)

solubility

H2O: 50 mg/mL

solubility

-

solubility

water: 5 mg/mL

anion traces

sulfate (SO42-): ≤50 mg/kg

anion traces

-

anion traces

-

anion traces

-

General description

Tris(2-carboxyethyl)-phosphine hydrochloride (TCEP) is a strong reducing agent that is stable in an aqueous solution. It readily and stoichiometrically reduces disulfides with high specificity. TCEP exhibits reducing activity at pH 1.5 to pH 9.0 and reduces dehydroascorbic acid (DHA) at pH 4.

Application

Tris(2-carboxyethyl)phosphine hydrochloride has been used:
  • as a component of IRE1 dialysis buffer, RNA cleavage buffer, and in in vitro cleavage assay of in vitro transcribed RNA
  • in reducing sulfhydryl groups to obtain total thiol fraction
  • to reduce protein samples

Water soluble reagent, used for selective reduction of disulfides. More stable than DTT and useful in mass spectrometry applications.

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 1


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