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75926

Oxonol VI

Name: Oxonol VI
Brand: SIGMA

suitable for fluorescence, ≥95% (UV)

Synonym(s):

1,5-Bis(5-oxo-3-propylisoxazol-4-yl)pentamethine oxonol

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About This Item

Empirical Formula (Hill Notation):

C₁₇H₂₀N₂O₄

CAS Number:

64724-75-0

Molecular Weight:

316.35

NACRES:

NA.32

PubChem Substance ID:

329766766

UNSPSC Code:

12171500

MDL number:

MFCD00210076

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assay

≥95% (UV)

form

powder

solubility

methanol: soluble

fluorescence

λ_ex 523 nm; λ_em 630 nm in 0.1 M Tris pH 9.0

suitability

suitable for fluorescence

shipped in

wet ice

storage temp.

−20°C

SMILES string

CCCC1=NOC(=O)\C1=C/C=C/C=C/c2c(O)onc2CCC

InChI

1S/C17H20N2O4/c1-3-8-14-12(16(20)22-18-14)10-6-5-7-11-13-15(9-4-2)19-23-17(13)21/h5-7,10-11,20H,3-4,8-9H2,1-2H3/b7-5+,10-6+,13-11-

InChI key

CPHARWHMAGUXDW-PQWGHOHWSA-N

Application

Oxonol VI can be used as a fluorescent potentiometric indicator to detect the membrane potentials in lipid vesicles. Quantitative measurements of the membrane potentials can be easily calibrated using oxonol VI because of its response to membrane potential changes. It is also used as an electric potential-sensitive probe to study membrane vesicles from E. coli cells.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

As an optical indicator for membrane potentials in lipid vesicles

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Ratiometric fluorescence measurements of membrane potential generated by yeast plasma membrane H+-ATPase reconstituted into vesicles

Holoubek A, et al.

Biochimica et Biophysica Acta - Biomembranes, 1609(1), 71-79 (2003)

Selective electrodiffusion of zinc ions in a Zrt-, Irt-like protein, ZIPB

Lin W, et al.

Journal of biological and chemical chronicles, 285(50), 39013-39020 (2010)

Preparation of Everted Membrane Vesicles from Escherichia coli Cells

Verkhovskaya M

Bio-protocol, 7(9), e2254-e2254 (2017)

H+ countertransport and electrogenicity of the sarcoplasmic reticulum Ca2+ pump in reconstituted proteoliposomes.

X Yu et al.

Biophysical journal, 64(4), 1232-1242 (1993-04-01)

The Ca2+ transport adenosine triphosphatase of sarcoplasmic reticulum was reconstituted in unilamellar liposomes prepared by reverse-phase evaporation. The size of the resulting proteoliposomes was similar to that of native sarcoplasmic reticulum vesicles, but their protein content was much lower, with

The effect of potential-sensitive molecular probes on the thermal phase transition in dimyristoylphosphatidylcholine preparations.

J Fumero et al.

Biochimica et biophysica acta, 944(2), 164-176 (1988-10-06)

Differential scanning calorimetry (DSC) has been employed to determine the effect of five commonly employed extrinsic potential-sensitive probes on phase transitions of multilamellar suspensions of L-alpha-dimyristoylphosphatidylcholine (DMPC). At mol% values of less than five, the effect of these probes on

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Global Trade Item Number

SKU	GTIN
W304522-1KG-K	04061838249166
W304522-SAMPLE-K	04061834405245
8210781000	04022536473420
8210780250	04022536473413
8210780005	04022536473406
W304522-5KG-K	04061838181732
W304522-10KG-K	04061838181725
75926-50MG	04061833278239

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