76216
Papain from Carica papaya
solution, light brown, ≥10 U/mg protein (~25 mg/ml)
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About This Item
CAS Number:
UNSPSC Code:
12352204
eCl@ss:
42030311
EC Number:
232-627-2
NACRES:
NA.54
MDL number:
Specific activity:
≥10 U/mg protein (~25 mg/ml)
Biological source:
papaya
biological source
papaya
Quality Segment
form
solution
specific activity
≥10 U/mg protein (~25 mg/ml)
technique(s)
cell culture | mammalian: suitable, single cell analysis: suitable
color
light brown
storage temp.
2-8°C
General description
Papain is a proteolytic enzyme and is naturally occurring in papaya (Carica papaya L.). It is obtained from the latex of cut unripe papaya. Papain belongs to the cysteine proteinase family. It is a globular, single-chain protein composed of four disulfide bridges and catalytically essential residues. Papain consists of an all α-domain and anti-parallel β-sheet domain.
Application
Papain from Carica papaya has been used:
- in the enzymatic dissociation of rat cortices to extract astrocytes for cell culture
- to study its effects on Alpaca seminal plasma viscosity and sperm function
- to study its effects on the yield and cryo-survival of Rhinoceros spermatozoa
Papain is used as a meat tenderizer. It′s also used to break up tissue in the preparation of primary cell cultures.
Biochem/physiol Actions
Papain plays an important role in many biological processes in many living organisms. It exhibits proteolytic activity against proteins, amino-acid esters and amide links, and short-chain peptides. Papain preferentially cleaves the peptide bonds of basic amino acids such as arginine, phenylalanine, and lysine. It has a wide range of applications in drug discovery, pharmaceutical, food, baking, and dairy industries.
Papain is a cysteine protease that cleaves peptide bonds and is known to cleave the Fc portion of antibodies.
Other Notes
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol N-benzoyl-L-arginine ethyl ester (BAEE, Cat. No. 12880) per minute at pH 6.2 and 25°C
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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