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Merck
CN

76258

Sigma-Aldrich

Paraplast Plus®

suitable for electron microscopy, pellets (fast-melting)

Synonym(s):

Paraplast Plus® embedding media, Paraffin – polyisobutylene mixture

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12171500
NACRES:
NA.47
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form

pellets (fast-melting)

Quality Level

technique(s)

electron microscopy: suitable

thickness

≤1 μm , short ribbons
≤2 μm , sectioning: continuous ribbons

mp

56-57 °C

application(s)

hematology
histology

storage temp.

2-8°C

General description

Paraplast Plus® is a refined mixture of highly purified paraffin containing complex elastomer of regulated molecular weights plus a small percentage of dimethyl sulfoxide (DMSO) for fast tissue penetration. It produces minimal tissue compression and wrinkle-free sections and cuts to 4 μm thickness with excellent ribbon continuity.
Paraplast® tissue embedding media are made from highly purified wax combined with plastic polymers for tensile strength and elasticity. Paraplast Plus® provides rapid infiltration during processing as well as great compression resistance during sectioning for preserved morphology and thin, distortion-free sections.
Our range of Paraplast products improve the elasticity of brittle, difficult-to-cut tissues making it easier to produce excellent, wrinkle-free sections.

Application

Paraffin polyisobutylene mixture is a tissue embedding medium composed of highly purified paraffin and polyisobutylene and a low level of dimethyl sulfoxide (DMSO) to enhance the rate of tissue penetration. Paraffin-polyisobutylene (76258) supports thin wrinkle-free sectioning down to 4 microM with more flexible and continuous ribbons.
Recommended for large specimens and tissue that commonly presents infiltration problems.

Features and Benefits

  • Produces minimal tissue compression and wrinkle-free sections, and cuts to 4 μm thickness with excellent ribbon continuity.
  • 0.8% DMSO enhances ease of infiltration and sectioning for tissues difficult to process with regular Paraplast.
  • Higher melt viscosity and lower permeability to water vapor creates better adhesion and a more pliable, flexible film.
  • Optimal infiltration and embedding for high-quality sections.
  • Great compression resistance and ribbon continuity.
  • Remarkable morphology preservation and distortion-free sections.

Legal Information

Paraplast is a registered trademark of Leica Biosystems
Paraplast Plus is a registered trademark of Leica Biosystems

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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