76258
Paraplast Plus®
suitable for electron microscopy, pellets (fast-melting)
Synonym(s):
Paraplast Plus® embedding media, Paraffin – polyisobutylene mixture
Product Name
Paraplast Plus®, suitable for electron microscopy, pellets (fast-melting)
form
pellets (fast-melting)
technique(s)
electron microscopy: suitable
thickness
≤1 μm , short ribbons
≤2 μm , sectioning: continuous ribbons
mp
56-57 °C
application(s)
hematology
histology
storage temp.
2-8°C
Quality Level
Application
Paraffin polyisobutylene mixture is a tissue embedding medium composed of highly purified paraffin and polyisobutylene and a low level of dimethyl sulfoxide (DMSO) to enhance the rate of tissue penetration. Paraffin-polyisobutylene (76258) supports thin wrinkle-free sectioning down to 4 microM with more flexible and continuous ribbons.
Recommended for large specimens and tissue that commonly presents infiltration problems.
Features and Benefits
- Produces minimal tissue compression and wrinkle-free sections, and cuts to 4 μm thickness with excellent ribbon continuity.
- 0.8% DMSO enhances ease of infiltration and sectioning for tissues difficult to process with regular Paraplast.
- Higher melt viscosity and lower permeability to water vapor creates better adhesion and a more pliable, flexible film.
- Optimal infiltration and embedding for high-quality sections.
- Great compression resistance and ribbon continuity.
- Remarkable morphology preservation and distortion-free sections.
General description
Paraplast Plus® is a refined mixture of highly purified paraffin containing complex elastomer of regulated molecular weights plus a small percentage of dimethyl sulfoxide (DMSO) for fast tissue penetration. It produces minimal tissue compression and wrinkle-free sections and cuts to 4 μm thickness with excellent ribbon continuity.
Paraplast® tissue embedding media are made from highly purified wax combined with plastic polymers for tensile strength and elasticity. Paraplast Plus® provides rapid infiltration during processing as well as great compression resistance during sectioning for preserved morphology and thin, distortion-free sections.
Our range of Paraplast products improve the elasticity of brittle, difficult-to-cut tissues making it easier to produce excellent, wrinkle-free sections.
Paraplast® tissue embedding media are made from highly purified wax combined with plastic polymers for tensile strength and elasticity. Paraplast Plus® provides rapid infiltration during processing as well as great compression resistance during sectioning for preserved morphology and thin, distortion-free sections.
Our range of Paraplast products improve the elasticity of brittle, difficult-to-cut tissues making it easier to produce excellent, wrinkle-free sections.
Legal Information
Paraplast is a registered trademark of Leica Biosystems
Paraplast Plus is a registered trademark of Leica Biosystems
Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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H O Goyal et al.
The Anatomical record, 250(2), 164-171 (1998-03-07)
Since androgens and/or estrogens must bind with specific receptors in order to elicit a response at the target organ(s), it is important to understand factors that regulate expression of androgen receptors (AR) and estrogen receptors (ER). Hence, the objective of
[Paraplast-plus, a tissue embedding medium that enables sections to stick to the slides without adhesives (author's transl)].
D Arn et al.
Mikroskopie, 31(3-4), 99-106 (1975-06-01)
H O Goyal et al.
The Anatomical record, 249(1), 54-62 (1997-09-19)
Because of the significance of androgens and estrogens in prenatal and postanatal differentiation of the testis and excurrent ducts, it is important to understand the developmental pattern of androgen receptor (AR) and estrogen receptor (ER) in these organs. Tissues from
Jing Qi et al.
Plant physiology, 147(4), 1947-1959 (2008-06-20)
The size and shape of the plant leaf is an important agronomic trait. To understand the molecular mechanism governing plant leaf shape, we characterized a classic rice (Oryza sativa) dwarf mutant named narrow leaf1 (nal1), which exhibits a characteristic phenotype
R S Liem et al.
Acta anatomica, 132(2), 164-168 (1988-01-01)
A method is described for the histochemical detection of horseradish peroxidase in Paraplast Plus embedded brain sections. The procedure uses 150-micron-thick Vibratome-cut slices of glutaraldehyde-paraformaldehyde-fixed brain tissue. Tetramethylbenzidine stabilized by diaminobenzidine/cobalt/H2O2 is used as chromogen. The Vibratome-cut slices are dehydrated
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