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Sigma-Aldrich

Peroxidase from horseradish

lyophilized, powder, ~150 U/mg

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Synonym(s):
(2R,3S,6S,7R,8R)-3-(3-formamido-2-hydroxybenzamido)-8-hexyl-2,6-dimethyl-4,9-dioxo-1,5-dioxonan-7-yl 3-methylbutanoate, Antimycin A, Horseradish peroxidase
CAS Number:
9003-99-0
Enzyme Commission number:
1.11.1.7 (BRENDA, IUBMB)
EC Number:
232-668-6
MDL number:
MFCD00071339
NACRES:
NA.54

biological source

horseradish

Quality Level

100

form

powder

quality

lyophilized

specific activity

~150 U/mg

mol wt

Mr ~40000

solubility

50 mM potassium phosphate buffer, pH 6.0: 5 mg/mL (0.1% BSA)

shipped in

wet ice

storage temp.

2-8°C

InChI

1S/H2O3/c1-3-2/h1-2H

InChI key

JSPLKZUTYZBBKA-UHFFFAOYSA-N

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General description

Horseradish peroxidase (HRP) is a vital and a widely studied heme-containing enzyme, isolated from a hardy perennial herb, horse radish. It catalyzes the oxidation of various organic and inorganic compounds by using hydrogen peroxide as substrates. HRP is involved in indole-3-acetic acid metabolism, cross-linking of biological polymers and lignification. This enzyme in combination with indole-3-acetic acid or its derivatives acts as a potential therapeutic for targeted cancer therapies. Commercially HRP is used as a constituent of clinical diagnostic kits and is used in immunoassays.

Application

Peroxidase from horseradish has been used:
  • for isolation of N-Linked glycans
  • as a constituent of D-amino acid oxidase (DAAO) buffer
  • to decompose hydrogen peroxide (H2O2) into water to demonstrate the performance of the portable oxidative stress sensor (POSS)

Horseradish peroxidase is used widely within biochemistry as a detection reagent in a variety of applications including ELISA, immunoblotting, and immunohistochemistry.

Unit Definition

1 U corresponds to the amount of enzyme which oxidizes 1 μmol ABTS (Cat. No. 11557) per minute at pH 6.0 and 25°C

Other Notes

Use of chemically modified peroxidase in benzene; Cross-linking of proteins; Enzyme catalyzed polymerization and precipitation of aromatic compounds from wastewater

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317 - H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Portable oxidative stress sensor: dynamic and non-invasive measurements of extracellular H2O2 released by algae
Koman VB, et al.
Biosensors And Bioelectronics, 68, 245-252 (2015)
Bacterial Competition Assay Based on Extracellular D-amino Acid Production
Alvarez, Laura and Cava, Felipe
The ISME Journal, 2018(3), 249-259 (2017)
K. Takahashi et al.
Biochemical and Biophysical Research Communications, 121, 216-216 (1984)
J.A. Nicell et al.
Water Science and Technology, 25, 157-157 (1992)
G. Matheis et al.
Journal of Protein Chemistry, 3, 35-35 (1984)
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