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Merck
CN

83636

Resorufin acetate

suitable for fluorescence, ≥98.0% (TLC)

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About This Item

Empirical Formula (Hill Notation):
C14H9NO4
CAS Number:
1152-14-3
Molecular Weight:
255.23
UNSPSC Code:
12352200
NACRES:
NA.32
PubChem Substance ID:
57652959
MDL number:
MFCD00037659
Beilstein/REAXYS Number:
246139

Key Documents

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Product Name

Resorufin acetate, suitable for fluorescence, ≥98.0% (TLC)

InChI

1S/C14H9NO4/c1-8(16)18-10-3-5-12-14(7-10)19-13-6-9(17)2-4-11(13)15-12/h2-7H,1H3

SMILES string

CC(=O)Oc1ccc2N=C3C=CC(=O)C=C3Oc2c1

InChI key

UJWKHDKBOVPINX-UHFFFAOYSA-N

assay

≥98.0% (TLC)

form

powder or crystals

mp

223 °C (lit.)

solubility

DMF: soluble
DMSO: soluble
alcohols: soluble

fluorescence

λex 500 nm; λem 593 nm in 0.1 M phosphate pH 8.0 (chymotrypsin)
λex 540 nm; λem 590 nm in DMSO
λex 571 nm; λem 584 nm (Reaction product)

suitability

suitable for fluorescence

storage temp.

2-8°C

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Application

suitable as fluorogenic substrate for hydrolytic enzymes (cellulases, chymotrypsin)

Other Notes

Studies on the esterase activity of cytosolic aldehyde dehydrogenase

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
BCCB4281
BCCB2742
BCBW6156
BCBP9343V
BCBN7819V

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Myung Gil Choi et al.
Organic letters, 12(7), 1468-1471 (2010-03-13)
The acetate derivatives of fluorescein and resorufin exhibited a prominent turn-on type signaling behavior toward BO(3)(-) ions over other common anions. Signaling is based on the selective deprotection of acetate groups by perborate, which resulted in significant chromogenic and fluorogenic
The action of cytosolic aldehyde dehydrogenase on resorufin acetate.
T M Kitson et al.
Advances in experimental medicine and biology, 414, 201-208 (1997-01-01)
T M Kitson
Biochimica et biophysica acta, 1385(1), 43-52 (1998-06-19)
Resorufin bromoacetate is a substrate that is rapidly hydrolysed by chymotrypsin. The reaction shows a pre-steady-state burst phase that may be observed by stopped flow spectrophotometry if precautions are taken against spontaneous hydrolysis of the substrate. The strongly activating effect
T M Kitson et al.
The Biochemical journal, 322 ( Pt 3), 701-708 (1997-03-15)
Resorufin acetate is a very good substrate for sheep liver cytosolic aldehyde dehydrogenase, both from the point of view of practical spectrophotometry and in terms of information provided about the nature of the catalysis shown by this enzyme. p-Nitrophenyl (PNP)
Mohit P Mathew et al.
Chembiochem : a European journal of chemical biology, 18(13), 1204-1215 (2017-02-22)
This report describes the metabolic glycoengineering (MGE) of intracellular esterase activity in human colon cancer (LS174T) and Chinese hamster ovary (CHO) cells. In silico analysis of carboxylesterases CES1 and CES2 suggested that these enzymes are modified with sialylated N-glycans, which
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