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83689

Rhodamine B

Name: Rhodamine B
Brand: SIGMA

suitable for fluorescence

Synonym(s):

Basic Violet 10, Brilliant Pink B, Rhodamine O, Tetraethylrhodamine

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About This Item

Empirical Formula (Hill Notation):

C₂₈H₃₁ClN₂O₃

CAS Number:

81-88-9

Molecular Weight:

479.01

EC Number:

201-383-9

UNSPSC Code:

12171500

NACRES:

NA.32

PubChem Substance ID:

57652962

MDL number:

MFCD00011931

Colour Index Number:

45170

Beilstein/REAXYS Number:

4091619

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form

powder or crystals

Quality Level

200

mol wt

479.01 g/mol

concentration

80-100 % (w/w) (Rhodamine B)

color

green to very dark green

mp

210-211 (dec.) (lit.)

solubility

H₂O: soluble, methanol: soluble

density

0.79 g/cm³

fluorescence

λ_ex 553 nm; λ_em 627 nm in methanol

suitability

in accordance for fluorescence, suitable for fluorescence

SMILES string

[Cl-].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccccc4C(O)=O)=[N+](/CC)CC)c1

InChI

1S/C28H30N2O3.ClH/c1-5-29(6-2)19-13-15-23-25(17-19)33-26-18-20(30(7-3)8-4)14-16-24(26)27(23)21-11-9-10-12-22(21)28(31)32;/h9-18H,5-8H2,1-4H3;1H

InChI key

PYWVYCXTNDRMGF-UHFFFAOYSA-N

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Clinical & Diagnostics

Dry Dyes & Certified Stains

Tissue Diagnostics

General description

Rhodamine B is a xanthene dye, which functions as a water tracer fluorescent. It is used as a staining fluorescent dye.

Rhodamine B is bright-red in color.

Application

Rhodamine B has been used:

as a fluorescent dye for cross staining 1-N-methylamino-5-isocyanonaphthalene (MICAN)
as a fluorescent dye in confocal microscopy
to study its fluorescence in water and ethanol solutions

for cross staining of N-methylamino-5-isocyanonaphthalene (MICAN)

Analysis Note

λ_max. ∼543 nm, E(1%/1cm) ∼2200-2800 (Methanol)

pictograms

GHS05,GHS07

signalword

Danger

hcodes

H302,H318,H412

pcodes

P264 - P273 - P280 - P301 + P312 - P305 + P351 + P338 - P501

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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The

A study of water circulation in Monterey harbor using rhodamine B dye (1965)

MICAN, a new fluorophore for vital and non-vital staining of human cells

Nagy Z, et al.

Toxicology in vitro, 48, 137-145 (2018)

Simplifying microfluidic separation devices towards field-detection of blood parasites

Holm SH, et al.

Analytical Methods : Advancing Methods and Applications, 8(16), 3291-3300 (2016)

The fluorescence properties of cationic rhodamine B in the gas phase

Sagoo SK and Jockusch RA

Journal of Photochemistry and Photobiology A: Chemistry, 220(2-3), 173-178 (2011)

Rhodamine B as a probe for phase transition in liquid solutions

Shishkin I, et al.

arXiv (2016)

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Global Trade Item Number

SKU	GTIN
83689-1G	04061833005774

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