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83702

Rhodamine 123

Name: Rhodamine 123
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥85% (HPLC)

Synonym(s):

2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester

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About This Item

Linear Formula:

C₂₁H₁₇ClN₂O₃

CAS Number:

62669-70-9

Molecular Weight:

380.82

UNSPSC Code:

12352116

NACRES:

NA.32

PubChem Substance ID:

57652966

EC Number:

263-687-8

Beilstein/REAXYS Number:

6030951

MDL number:

MFCD00012664

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product line

BioReagent

Quality Level

100

assay

≥85% (HPLC)

form

powder

impurities

≤10% water

solubility

ethanol: 20 mg/mL, clear, red

suitability

in accordance for fluorescence, suitable for fluorescence

SMILES string

Cl[H].COC(=O)c1ccccc1C2=C3C=CC(=N)C=C3Oc4cc(N)ccc24

InChI

1S/C21H16N2O3.ClH/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20;/h2-11,22H,23H2,1H3;1H

InChI key

MYFATKRONKHHQL-UHFFFAOYSA-N

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Clinical & Diagnostics

Dry Dyes & Certified Stains

Tissue Diagnostics

General description

Rhodamine 123 is a fluorescent dye.

Application

Rhodamine 123 has been used:

as a substrate for P-glycoprotein (P-gp) to read out functional transporter activity in fluorescent accumulation assays
to label the cells for estimating the P-gp expression by flow cytometry
in transport studies, was added to the apical to basolateral (AB-transport) or basolateral to apical (BA-transport)
the detection of mitochondrial membrane potential (ΔΨm)

Biochem/physiol Actions

Rhodamine 123 is usually used as a functional reporter for P-glycoprotein (P-gp) in flow cytometry.

Fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). Recent reports indicate Rh 123 may also be a substrate of MRP1.

Other Notes

Supravital mitochondrial-specific dye

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Mitochondrial analysis in living cells: the use of rhodamine 123 and flow cytometry.

X Ronot et al.

Biology of the cell, 57(1), 1-7 (1986-01-01)

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Global Trade Item Number

SKU	GTIN
83702-50MG	04061833005798
83702-10MG	04061833005781

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