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About This Item
NACRES:
NA.81
UNSPSC Code:
41106514
Biological source:
Mouse x Mouse T cell Hybridoma
Technique(s):
cell culture | mammalian: suitable
Myeloma:
Azaguanine resistant BW5147 AKR mouse tumour cells
Reactivity:
T cell hybridoma
biological source
Mouse x Mouse T cell Hybridoma
Quality Level
form
liquid
packaging
tube of 5 μg 85082303-DNA-5UG, pkg of vial of cells 85082303-1VL
ImmunoDon
B6DF mouse T cells
Myeloma
Azaguanine resistant BW5147 AKR mouse tumour cells
Reactivity
T cell hybridoma
technique(s)
cell culture | mammalian: suitable
Biochem/physiol Actions
This cell line is a stable T cell hybridoma which, when stimulated with concanavalin A, produces IL-2. The cell surface markers include Thy1.2 and H-2b products.
Packaging
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
Preparation Note
Maintain cultures between 2-6 x 100,000 cells/ml; 5% CO2; 37 °C.
RPMI 1640 + 2 mM glutamine + 1 mM Sodium Pyruvate (NaP) + 10% Horse Serum (HS).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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L Harwell et al.
The Journal of experimental medicine, 152(4), 893-904 (1980-10-01)
The fusion of an AKR T cell tumor line to normal B6D2F1, T cells resulted in the production of a cloned T cell hybridoma (FS6-14.13) inducible with the mitogen concanavalin A (Con A). The supernate from Con A-stimulated hybridoma cells
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 85082303-1VL | 04061837344664 |