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Merck
CN

85082303

FS6-14.13 Ag2.1 cell line

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA

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关于此项目

NACRES:
NA.81
UNSPSC Code:
41106514
Biological source:
Mouse x Mouse T cell Hybridoma
Technique(s):
cell culture | mammalian: suitable
Myeloma:
Azaguanine resistant BW5147 AKR mouse tumour cells
Reactivity:
T cell hybridoma
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biological source

Mouse x Mouse T cell Hybridoma

Quality Level

form

liquid

packaging

tube of 5 μg 85082303-DNA-5UG, pkg of vial of cells 85082303-1VL

ImmunoDon

B6DF mouse T cells

Myeloma

Azaguanine resistant BW5147 AKR mouse tumour cells

Reactivity

T cell hybridoma

technique(s)

cell culture | mammalian: suitable

Biochem/physiol Actions

This cell line is a stable T cell hybridoma which, when stimulated with concanavalin A, produces IL-2. The cell surface markers include Thy1.2 and H-2b products.

Packaging

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Preparation Note

Maintain cultures between 2-6 x 100,000 cells/ml; 5% CO2; 37 °C.
RPMI 1640 + 2 mM glutamine + 1 mM Sodium Pyruvate (NaP) + 10% Horse Serum (HS).

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.


存储类别

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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L Harwell et al.
The Journal of experimental medicine, 152(4), 893-904 (1980-10-01)
The fusion of an AKR T cell tumor line to normal B6D2F1, T cells resulted in the production of a cloned T cell hybridoma (FS6-14.13) inducible with the mitogen concanavalin A (Con A). The supernate from Con A-stimulated hybridoma cells



全球贸易项目编号

货号GTIN
85082303-1VL04061837344664