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5-Carboxyfluorescein

Name: 5-Carboxyfluorescein
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥95% (HPLC)

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Synonym(s):

5-FAM

Empirical Formula (Hill Notation):

C₂₁H₁₂O₇

CAS Number:

76823-03-5

Molecular Weight:

376.32

Beilstein:

57037

MDL number:

MFCD00036874

PubChem Substance ID:

57653195

product line

BioReagent

Quality Level

100

Assay

≥95% (HPLC)

form

powder

solubility

DMF: soluble
DMSO: soluble
H₂O: soluble

fluorescence

λ_ex 492 nm; λ_em 517 nm in 0.1 M Tris pH 8.0

suitability

suitable for fluorescence

SMILES string

OC(=O)c1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35

InChI

1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)

InChI key

NJYVEMPWNAYQQN-UHFFFAOYSA-N

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General description

5-Carboxyfluorescein, also known as 5-FAM, is a green fluorescent product. It is derived from cleavage of 5-Carboxyfluorescein diacetate by intracellular esterases. In viable cells, the fluorescent molecule is impermeable to the cellular membrane.

Application

5-Carboxyfluorescein has been used as a payload to study leakage phenomena on sesame (Sesamum indicum L.) and coconut (Cocos nucifera L.) liposomes.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Derivative of fluorescein giving stable derivative upon conjugation with biopolymers

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Intracellular pH and its Measurement (1989)

mRNA processing in Antonospora locustae spores.

Nicolas Corradi et al.

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Study on leakage of sesame (Sesamum indicum L.) and coconut (Cocos nucifera L.) liposomes

Hudiyanti D, et al.

Orient. J. Chem., 31, 435-439 (2015)

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