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87444

5-Carboxy-fluorescein diacetate N-succinimidyl ester

Name: 5-Carboxy-fluorescein diacetate <I>N</I>-succinimidyl ester
Brand: SIGMA

suitable for fluorescence, ≥95.0% (HPLC)

Synonym(s):

5-Carboxy-di-O-acetylfluorescein N-succinimidyl ester

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About This Item

Empirical Formula (Hill Notation):

C₂₉H₁₉NO₁₁

CAS Number:

150206-05-6

Molecular Weight:

557.46

NACRES:

NA.32

PubChem Substance ID:

57653248

UNSPSC Code:

12352108

MDL number:

MFCD00037466

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Quality Level

100

assay

≥95.0% (HPLC)

form

powder

solubility

DMF: soluble, DMSO: soluble

fluorescence

λ_ex 492 nm; λ_em 517 nm

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(=O)ON6C(=O)CCC6=O)c1.CC(=O)Oc7ccc8c(Oc9cc(OC(C)=O)ccc9C8%10OC(=O)c%11ccc(cc%10%11)C(=O)ON%12C(=O)CCC%12=O)c7

InChI

1S/2C29H19NO11/c1-14(31)37-17-4-7-21-23(12-17)39-24-13-18(38-15(2)32)5-8-22(24)29(21)20-6-3-16(11-19(20)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34;1-14(31)37-17-4-7-20-23(12-17)39-24-13-18(38-15(2)32)5-8-21(24)29(20)22-11-16(3-6-19(22)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h2*3-8,11-13H,9-10H2,1-2H3

InChI key

LZFDYQHOQDFMOA-UHFFFAOYSA-N

Other Notes

Continuous determination of the intracellular pH in bacteria by fluorometry; labeling human intervertebral disk cells in vitro for fluorescence microscopy

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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A Novel Method for Continuous Determination of the Intracellular pH in Bacteria with the Internally Conjugated Fluorescent Probe 5 (and 6-)-Carboxyfluorescein Succinimidyl Ester.

P Breeuwer et al.

Applied and environmental microbiology, 62(1), 178-183 (1996-01-01)

A novel method based on the intracellular conjugation of the fluorescent probe 5 (and 6-)-carboxyfluorescein succinimidyl ester (cFSE) was developed to determine the intracellular pH of bacteria. cFSE can be taken up by bacteria in the form of its diacetate

Optimization of 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester for labeling human intervertebral disc cells in vitro.

H E Gruber et al.

Biotechnic & histochemistry : official publication of the Biological Stain Commission, 75(3), 118-123 (2000-08-19)

We have assessed the utility of an intracellular fluorochrome, 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester (CFSE), as a tracking label for human intervertebral disc cells in vitro. Although 5 microM provides adequate intracellular labeling for whole cell fluorescent microscopic identification of labeled

Lymph node stromal cells acquire peptide-MHCII complexes from dendritic cells and induce antigen-specific CD4⁺ T cell tolerance.

Juan Dubrot et al.

The Journal of experimental medicine, 211(6), 1153-1166 (2014-05-21)

Dendritic cells (DCs), and more recently lymph node stromal cells (LNSCs), have been described to tolerize self-reactive CD8(+) T cells in LNs. Although LNSCs express MHCII, it is unknown whether they can also impact CD4(+) T cell functions. We show

Fatal autoimmunity in mice reconstituted with human hematopoietic stem cells encoding defective FOXP3.

Jeremy A Goettel et al.

Blood, 125(25), 3886-3895 (2015-04-03)

Mice reconstituted with a human immune system provide a tractable in vivo model to assess human immune cell function. To date, reconstitution of murine strains with human hematopoietic stem cells (HSCs) from patients with monogenic immune disorders have not been

Inhibition of Akt signaling promotes the generation of superior tumor-reactive T cells for adoptive immunotherapy.

Anniek B van der Waart et al.

Blood, 124(23), 3490-3500 (2014-10-23)

Effective T-cell therapy against cancer is dependent on the formation of long-lived, stem cell-like T cells with the ability to self-renew and differentiate into potent effector cells. Here, we investigated the in vivo existence of stem cell-like antigen-specific T cells

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Global Trade Item Number

SKU	GTIN
87444-5MG-F	04061832533421

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