Skip to Content
Merck
CN

87961

Sigma-Aldrich

Tetranitroblue tetrazolium chloride

≥85% (AT)

Synonym(s):

3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2,5-bis(p-nitrophenyl)-2H-tetrazolium chloride], TNBT, Tetranitrotetrazolium blue chloride

Sign Into View Organizational & Contract Pricing

Select a Size

About This Item

Empirical Formula (Hill Notation):
C40H28Cl2N12O10
CAS Number:
1184-43-6
Molecular Weight:
907.63
Beilstein:
3897475
EC Number:
214-665-1
MDL number:
MFCD00036338
UNSPSC Code:
12352204
PubChem Substance ID:
57653280
NACRES:
NA.83

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Assay

≥85% (AT)

form

powder or crystals

ign. residue

≤0.1%

loss

≤25% loss on drying

mp

~170 °C (dec.)

solubility

2-methoxyethanol: 0.090 g/10 mL (hot)

SMILES string

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccc(cc4)[N+]([O-])=O)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccc(cc8)[N+]([O-])=O

InChI

1S/C40H28N12O10.2ClH/c1-61-37-23-27(7-21-35(37)47-43-39(25-3-9-31(10-4-25)49(53)54)41-45(47)29-13-17-33(18-14-29)51(57)58)28-8-22-36(38(24-28)62-2)48-44-40(26-5-11-32(12-6-26)50(55)56)42-46(48)30-15-19-34(20-16-30)52(59)60;;/h3-24H,1-2H3;2*1H/q+2;;/p-2

InChI key

VCESGVLABVSDRO-UHFFFAOYSA-L

Looking for similar products? Visit Product Comparison Guide

General description

crystallized with ~2 mol ethanol

Other Notes

Review: Tetrazolium salts and formazans; Detection of hydrolases (with indoxyl substrates)

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H350

Precautionary Statements

P201 - P308 + P313

Hazard Classifications

Carc. 1B

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000144445
0000095640
0000089114
0000069420

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

F.P. Altman
Progress in Histochemistry and Cytochemistry, 9, 51-51 (1976)
R Gossrau
Histochemistry, 58(3), 203-218 (1978-12-01)
Using freeze-dried or sections from fresh-frozen or aldehyde-fixed material nitro BT (NBT), tetranito BT (TNBT), distyryl nitro BT (DS-NBT), thiocarbamyl nitro BT (TC-NBT) or benzothiazolylstyrylphthalhydrazidyl tetrazolium chloride (BSPT) were tested as auxiliary reagents for the localization of glycosidases, phosphatases and
W M Frederiks et al.
The Histochemical journal, 17(6), 707-715 (1985-06-01)
The tetrazolium method for the histochemical detection of monoamine oxidase (MAO) activity in rat liver cryostat sections has been tested for its specificity and its possible use in quantification. The tetrazolium salt tetranitro blue tetrazolium is recommended for the localization
D J Wigston
The Journal of neuroscience : the official journal of the Society for Neuroscience, 9(2), 639-647 (1989-02-01)
The stability of neuromuscular junctions (NMJs) was studied in soleus muscles of adult mice by labeling acetylcholine receptors in vivo with rhodamine alpha-bungarotoxin. Identified NMJs were examined in living animals by low-light-level fluorescence microscopy on 2 or 3 occasions separated
C J Van Noorden et al.
The Histochemical journal, 19(2), 94-102 (1987-02-01)
The indoxyl-tetranitro BT method for the demonstration of alkaline phosphatase activity has been optimized and its validity for quantitative histochemistry tested. The study has been performed with model films of polyacrylamide gel incorporating homogenate of rat liver and with cryostat
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service