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Merck
CN

88596

Fluorescein-O′-acetic acid

BioReagent, suitable for fluorescence, ≥90% (HPCE)

Synonym(s):

FOAA, O′-(Carboxymethyl)fluorescein

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About This Item

Empirical Formula (Hill Notation):
C22H14O7
CAS Number:
233759-98-3
Molecular Weight:
390.34
NACRES:
NA.32
PubChem Substance ID:
57653272
UNSPSC Code:
12352106
MDL number:
MFCD05865263

Key Documents

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InChI

1S/C22H14O7/c23-12-5-7-16-18(9-12)29-19-10-13(28-11-20(24)25)6-8-17(19)21(16)14-3-1-2-4-15(14)22(26)27/h1-10H,11H2,(H,24,25)(H,26,27)

SMILES string

OC(=O)COc1ccc2c(OC3=CC(=O)C=CC3=C2c4ccccc4C(O)=O)c1

InChI key

VQKDLLIKXLDPHW-UHFFFAOYSA-N

product line

BioReagent

assay

≥90% (HPCE)

form

powder

fluorescence

λex 455 nm; λem 514 nm in 0.1 M Tris pH 8.0

suitability

suitable for fluorescence

Quality Level

100

Other Notes

Fluorescent label which reacts with amines after activation to give isomerically homogeneous derivatives

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
449284/1

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Analytica Chimica Acta, 423, 77-77 (2000)
H Wang et al.
Analytical biochemistry, 281(1), 15-20 (2000-06-10)
A new amine-reactive derivatizing reagent, N-hydroxysuccinimidyl fluorescein-O-acetate (SIFA), was developed for catecholamine (CA) analysis in liquid chromatography. The reactivity of this reagent with the CAs norepinephrine (NE), epinephrine (E), and dopamine (DA) was investigated in detail. In aqueous methanol containing

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