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92846

5-Carboxyfluorescein N-succinimidyl ester

Name: 5-Carboxyfluorescein <I>N</I>-succinimidyl ester
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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About This Item

Empirical Formula (Hill Notation):

C₂₅H₁₅NO₉

CAS Number:

92557-80-7

Molecular Weight:

473.39

UNSPSC Code:

12352108

NACRES:

NA.32

PubChem Substance ID:

57653496

MDL number:

MFCD00077322

Beilstein/REAXYS Number:

7865828

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Properties

product line

BioReagent

Quality Level

100

assay

≥90% (HPLC)

form

powder

fluorescence

λ_ex 492 nm; λ_em 520 nm in 0.1 M Tris pH 8.0

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

OC(=O)c1cc(ccc1C2=C3C=CC(=O)C=C3Oc4cc(O)ccc24)C(=O)ON5C(=O)CCC5=O

InChI

1S/C25H15NO9/c27-13-2-5-16-19(10-13)34-20-11-14(28)3-6-17(20)23(16)15-4-1-12(9-18(15)24(31)32)25(33)35-26-21(29)7-8-22(26)30/h1-6,9-11,27H,7-8H2,(H,31,32)

InChI key

TWOKNYRPKCLMAR-UHFFFAOYSA-N

Description

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Derivative of fluorescein giving stable derivative upon conjugation with biopolymers. Fluorescent probe for nucleotides; Reagent for fluorescent derivatization in capillary electrophoresis

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Nonradioactive labeling of RNA.

G L Igloi

Analytical biochemistry, 233(1), 124-129 (1996-01-01)

A modified ribodinucleotide (named pSEEp) has been synthesized using commercially available components on a DNA synthesizer. The presence of a 3'-terminal primary amino group permits its coupling to a range of nonradioactive labels, exemplified here by fluorescein. The product of

Lau, S.K., et al.

Journal of Chromatography A, 809, 203-203 (1998)

Monocyte adhesion to atherosclerotic matrix proteins is enhanced by Asn-Gly-Arg deamidation.

Bamaprasad Dutta et al.

Scientific reports, 7(1), 5765-5765 (2017-07-20)

Atherosclerosis arises from leukocyte infiltration and thickening of the artery walls and constitutes a major component of vascular disease pathology, but the molecular events underpinning this process are not fully understood. Proteins containing an Asn-Gly-Arg (NGR) motif readily undergo deamidation

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Global Trade Item Number

SKU	GTIN
92846-5MG-F	04061833270486