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Merck
CN

93145

1,3,5-Triphenyltetrazolium formazan

≥90% (UV)

Synonym(s):

TTC formazan

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About This Item

Empirical Formula (Hill Notation):
C19H16N4
CAS Number:
Molecular Weight:
300.36
UNSPSC Code:
12352204
NACRES:
NA.83
PubChem Substance ID:
EC Number:
208-509-1
Beilstein/REAXYS Number:
753057
MDL number:
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Quality Segment

assay

≥90% (UV)

form

powder

solubility

dioxane: 50 mg/mL, clear

SMILES string

N(\N=C(/N=N/c1ccccc1)c2ccccc2)c3ccccc3

InChI

1S/C19H16N4/c1-4-10-16(11-5-1)19(22-20-17-12-6-2-7-13-17)23-21-18-14-8-3-9-15-18/h1-15,20H/b22-19-,23-21+

InChI key

BEIHVSJTPTXQGB-YMGPPEANSA-N

General description

1,3,5-Triphenyltetrazolium formazan is an insoluble compound produced upon the reduction of 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) salt, which is an indicator of cell redox potential. This reaction is irreversible. TTC formazan is brightly colored and can be quantified using a spectrophotometer.

Application

1,3,5-Triphenyltetrazolium formazan has been used:
  • for the quantification of 2,3,5-triphenyltetrazolium chloride (TTC) reduction activity in bacterial cells
  • as a standard to study the optical density of formazan produced by TTC-treated milled seeds
  • to stain heart tissue for infarct size assessment or undergo perfusion fixation to allow morphometric studies in addition to histology and immunohistochemistry

Other Notes

Review: Tetrazolium salts and formazans


pictograms

Exclamation markEnvironment

signalword

Warning

hcodes

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves



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