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Merck
CN

93773

TWEEN® 20

viscosity 250-450 mPa.s (25 °C)

Synonym(s):

Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate

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About This Item

CAS Number:
9005-64-5
UNSPSC Code:
12161900
NACRES:
NA.21
MDL number:
MFCD00165986

Key Documents

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description

non-ionic

form

viscous liquid

Quality Level

200

technique(s)

DNA extraction: suitable, RNA extraction: suitable, cell culture | mammalian: suitable, protein purification: suitable

refractive index

n20/D 1.468 (lit.), n20/D 1.471

viscosity

250-450 mPa.s(25 °C)

HLB value

16.7±1.0

density

1.11 g/mL at 20 °C

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General description

Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

Application

Non-ionic detergent
Tween® 20 is widely used in several biochemical applications. It has been used:

  • as an emulsifying agent for the preparation of stable oil-in-water emulsions
  • in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)
  • as a blocking agent for membrane based immunoassays at a typical concentration of 0.05% - 1%
  • for lysing mammalian cells at a concentration of 0.005 to 0.5%
  • for rehydrating tissue before in situ hybridization

Biochem/physiol Actions

Tween® 20, functioning as a detergent, potentially operates by binding to the hydrophobic components of water-insoluble substances, thereby making them hydrophilic.

Features and Benefits

  • Suitable for protein purification and DNA and RNA extraction
  • Suitable for mammalian cell culture
  • High purity product for research applications

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Disclaimer

may get turbid on storage, heat to clear

Storage Class

10 - Combustible liquids

flash_point_f

527.0 °F - Pensky-Martens closed cup

flash_point_c

275 °C - Pensky-Martens closed cup

ppe

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Certificates of Analysis (COA)

Lot/Batch Number
BCCP1332
BCCN8382
BCCN5133
BCCN0803
BCCN0328

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Neugebauer JM
Methods in Enzymology, 182, 239-253 (1990)
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