94149
Atto 647N-Streptavidin
BioReagent, suitable for fluorescence
Synonym(s):
Streptavidin-Atto 647N
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About This Item
NACRES:
NA.25
UNSPSC Code:
12161900
product line
BioReagent
form
powder
manufacturer/tradename
ATTO-TEC GmbH
λ
in ethanol (with 0.1% trifluoroacetic acid)
UV absorption
λ: 650.0-656.0 nm Amax
suitability
corresponds for gel electrophoresis, suitable for fluorescence
storage temp.
−20°C
Quality Level
Related Categories
General description
Atto 647N is a new generation fluorescent dye dedicated towards the red spectral region. It is a cationic dye and carries a net electrical charge of +1 post substrate coupling reaction. Streptavidin, isolated from Streptomyces avidinii, is a tetrameric protein of 4 x 13.2 kDa which binds very tightly to the small molecule biotin. The dissociation constant of the complex is extremely small (Kd ≈ 10-15 M), ranking among the strongest non-covalent interactions. Atto streptavidin conjugates are readily soluble in water.
Application
Atto 647N-Streptavidin is designed for application in the area of life science, e.g., labeling of DNA, RNA or proteins. Atto streptavidin conjugates may be used as secondary detection reagents in flow cytometry, immunoassays, blot analysis, and histochemical applications. Atto 647N -Streptavidin is suitable for multiplex analysis, including multicolor imaging.
Features and Benefits
Atto 647N- Streptavidin has the following benefits-
- Highly photostable. Strong absorption.
- Excellent fluorescent quantum yield.
- Highly resistant to ozone degradation.
- Minimal triplet formation.
Analysis Note
dye-to-protein ratio ≥1.5
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Articles
Atto dyes are a series of fluorescent dyes that meet the critical needs of modern fluorescent technologies.
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