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Merck
CN

94281

Urease from Canavalia ensiformis (Jack bean)

powder, white, ~8 U/mg

Synonym(s):

Jack bean urease, Urea amidohydrolase

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About This Item

CAS Number:
eCl@ss:
32160410
UNSPSC Code:
12352204
EC Number:
232-656-0
NACRES:
NA.54
MDL number:
EC Number:
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Product Name

Urease from Canavalia ensiformis (Jack bean), powder, white, ~8 U/mg

biological source

Canavalia ensiformis

form

powder

specific activity

~8 U/mg

mol wt

Mr ~480000

color

white

storage temp.

−20°C

Quality Level

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Application

Urease from Canavalia ensiformis may be used for urea determination of various samples, such as legumes . It may be useful for the detection of pathogens as well as heavy-metal ions.

Biochem/physiol Actions

Urease catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is involved in nitrogen metabolism and urea degradation. Urease from Canavalia ensiformis binds 2 nickel ions per subunit .

General description

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Urease (Ure) enzyme is present in the cytosol. It is active at acidic pH. Urease is present in archaea, bacteria, unicellular eukaryotes and plants.

Other Notes

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol urea (Cat. No. 51459) per minute at pH 8.0 and 25°C

pictograms

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signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 1

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Regulatory Information

动植物源性产品
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Yersinia enterocolitica
Molecular Medical Microbiology, 1319-1344 (2015)
Isothermal titration calorimetry to characterize enzymatic reactions
Test, 567, 215-236 (2016)
Jesus M Duran Ramirez et al.
Frontiers in cellular and infection microbiology, 12, 859093-859093 (2022-04-09)
Catheter-associated urinary tract infections (CAUTIs) are one of the most common healthcare-associated infections in the US, accounting for over 1 million cases annually and totaling 450 million USD. CAUTIs have high morbidity and mortality rates and can be caused by
Alfonso Muñoz et al.
Journal of experimental botany, 62(1), 307-318 (2010-09-04)
Ureidoglycolate is an intermediate in the degradation of the ureides, allantoin and allantoate, found in many organisms. In some leguminous plant species these compounds are used to transport recently fixed nitrogen in the root nodules to the aerial parts of
Tomoyo Matsushita Yamamoto et al.
Medical molecular morphology, 45(4), 199-205 (2012-12-12)
Electrolyzed acid water (EAW) has been studied for its disinfective potential against pathogenic microbes; however, the bactericidal process against Mycobacteria has not been clearly presented. In this study, to clarify the disinfective process against Mycobacteria, EAW-treated bacteria were examined against

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