94281
Urease from Canavalia ensiformis (Jack bean)
powder, white, ~8 U/mg
Synonym(s):
Jack bean urease, Urea amidohydrolase
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About This Item
CAS Number:
eCl@ss:
32160410
UNSPSC Code:
12352204
EC Number:
232-656-0
NACRES:
NA.54
MDL number:
Specific activity:
~8 U/mg
Biological source:
Canavalia ensiformis
biological source
Canavalia ensiformis
Quality Segment
form
powder
specific activity
~8 U/mg
mol wt
Mr ~480000
color
white
storage temp.
−20°C
General description
Urease (Ure) enzyme is present in the cytosol. It is active at acidic pH. Urease is present in archaea, bacteria, unicellular eukaryotes and plants.
Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Composed of six subunits with total molecular weight: ~544,620
Application
Urease from Canavalia ensiformis may be used for urea determination of various samples, such as legumes . It may be useful for the detection of pathogens as well as heavy-metal ions.
Biochem/physiol Actions
Urease catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is involved in nitrogen metabolism and urea degradation. Urease from Canavalia ensiformis binds 2 nickel ions per subunit .
Other Notes
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol urea (Cat. No. 51459) per minute at pH 8.0 and 25°C
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 1
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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