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Merck
CN

94310

Uricase from Bacillus fastidiosus

lyophilized, ~9 U/mg

Synonym(s):

Urate oxidase

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About This Item

CAS Number:
9002-12-4
UNSPSC Code:
12352204
NACRES:
NA.54
PubChem Substance ID:
57653596
MDL number:
MFCD00082126
EC Number:
1.7.3.3 (BRENDA, IUBMB)

Key Documents

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Product Name

Uricase from Bacillus fastidiosus, lyophilized, ~9 U/mg

InChI

1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3

SMILES string

N.OCC1OC(C(O)C1OP(O)(=O)OCC2OC(C(O)C2O)N3C=CC(=O)NC3=O)N4C=CC(=O)NC4=O

InChI key

VPXDHZMLJOJKOX-UHFFFAOYSA-N

biological source

Bacillus sp. (B. fastidiosus)

form

powder

quality

lyophilized

specific activity

~9 U/mg

storage condition

dry at room temperature

concentration

90—100%

color

white to faint beige

solubility

5 mg/mL, clear, colorless to very faintly yellow (in enzyme dilution buffer)

application(s)

detection

storage temp.

−20°C

Quality Level

300

Application

Uricase may be used for the determination of uric acid in biological fluids. It may be helpful in the spectrophotometric determination of purines and purine nucleosides.

Biochem/physiol Actions

Uricase catalyzes the oxidation of uric acid to 5-hydroxyisourate, which subsequently forms (S)-allantoin. It is involved in purine metabolism, urate degradation and (S)-allantoin formation The absence of functional uricase in humans has significant physiological and pathophysiological implications. It has been associated with higher serum uric acid levels, leading to an increased risk of conditions related to uric acid crystallization, such as gout. Uricase has emerged as an important therapeutic target for the treatment of conditions related to hyperuricemia, such as gout.

General description

Research area: Cell signaling
Uricase is present in many organisms, such as, archaea, bacteria, and eukaryotes. The Gut microbiome is the major source of uricase, a homotetrameric enzyme containing four identical 34 kDa subunits.

Other Notes

1 U corresponds to the amount of enzyme which oxidizes 1 μmole uric acid at pH 9.1 and 37 °C.
For the determination of uric acid in biological fluids; In the spectrophotometric determination of purines and purine nucleosides.

pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Resp. Sens. 1

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

常规特殊物品
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Certificates of Analysis (COA)

Lot/Batch Number
BCCN8035
BCCM8800
BCCL4872
BCCM5301
BCCL0755

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U. Naegele et al.
Methods of Enzymatic Analysis, 7, 140-140 (1985)
A Therapeutic Uricase with Reduced Immunogenicity Risk and Improved Development Properties
Nyborg AC, et al.
PLoS ONE (2016)
Youssef M Roman
Journal of personalized medicine, 13(9) (2023-09-28)
Uric acid is the final product of purine metabolism and is converted to allantoin in most mammals via the uricase enzyme. The accumulation of loss of function mutations in the uricase gene rendered hominoids (apes and humans) to have higher
Berna Dalkıran et al.
Mikrochimica acta, 187(11), 609-609 (2020-10-16)
Electropolymerization of thionine (TH) on multiwalled carbon nanotube (MWCNT)-modified glassy carbon electrodes (GCE) in ethaline deep eutectic solvent (DES) was carried out for the first time, to prepare poly(thionine) (PTH) films with different nanostructured morphologies. PTH films were formed on
M.H. Jensen et al.
Methods of Enzymatic Analysis, 7, 101-101 (1985)
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