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Merck
CN

94498

5-Cyano-2,3-di-(p-tolyl)tetrazolium chloride

≥85% (HPLC)

Synonym(s):

5-Cyano-2,3-bis(4-methylphenyl)-2H-tetrazolium chloride, CTC

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About This Item

Empirical Formula (Hill Notation):
C16H14ClN5
CAS Number:
Molecular Weight:
311.77
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
8954451
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Quality Level

assay

≥85% (HPLC)

form

powder

storage temp.

2-8°C

SMILES string

[Cl-].Cc1ccc(cc1)-n2nc(n[n+]2-c3ccc(C)cc3)C#N

InChI

1S/C16H14N5.ClH/c1-12-3-7-14(8-4-12)20-18-16(11-17)19-21(20)15-9-5-13(2)6-10-15;/h3-10H,1-2H3;1H/q+1;/p-1

InChI key

SSJZAXOTLCJNLF-UHFFFAOYSA-M

General description

5-cyano-2,3-di-(p-tolyl)-tetrazolium chloride is a Redox Sensitive tetrazolium salt producing a fluorescent formazan (only in solid state) upon reduction; It indicates the respiratory activity in cells.

Application

5-cyano-2,3-di(p-tolyl) tetrazolium chloride (CTC)-reduction test is used for live/dead analysis. 5-cyano-2,3-di-(p-tolyl)-tetrazolium chloride has been employed in histochemistry and for determining cell viability, for eukaryotic cell viability assays and bacterial viability experiments, 5-cyano-2,3-di-(p-tolyl)-tetrazolium chloride (CTC) is the most often employed compound.


pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves



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Juan C Stockert et al.
Acta histochemica, 120(3), 159-167 (2018-03-03)
For many years various tetrazolium salts and their formazan products have been employed in histochemistry and for assessing cell viability. For the latter application, the most widely used are 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), and 5-cyano-2,3-di-(p-tolyl)-tetrazolium chloride (CTC) for viability assays of
Rodriguez, G.G., et al.
Applied and Environmental Microbiology, 58, 180-180 (1992)
Severin, E., et al.
Analytica Chimica Acta, 170, 341-341 (1985)



Global Trade Item Number

SKUGTIN
94498-10MG-F04061832534022
94498-50MG-F04061833295663