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95284

Water

Name: Water
Brand: SIGMA

for molecular biology, suitable for FISH

Synonym(s):

H₂O

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About This Item

Linear Formula:

H₂O

CAS Number:

7732-18-5

Molecular Weight:

18.02

NACRES:

NA.31

PubChem Substance ID:

57653665

UNSPSC Code:

12191602

EC Number:

231-791-2

MDL number:

MFCD00011332

Beilstein/REAXYS Number:

2050024

Grade:

Molecular Biology

Technique(s):

FISH: suitable

Bp:

100 °C (lit.)

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Product Name

Water, Molecular Biology, sterile filtered

grade

Molecular Biology

Quality Level

100

sterility

sterile-filtered

form

liquid

quality

sterile filtered

shelf life

limited shelf life, expiry date on the label

technique(s)

FISH: suitable

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

refractive index

n20/D 1.34 (lit.)

pH

5-7

conductivity

0.0-5 μS/cm at 25 °C

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

cation traces

Ca: <10 ppb, Mg: <10 ppb

SMILES string

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

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General description

Molecular biology grade water is sterile filtered and ready to use, free from DNase, RNase, phosphatase, and protease contamination.

Application

Water, sterile filtered has been used:

for cloning and plasmid construction.
for restriction digestion of PCR amplicons in polysaccharide A (PSA) restriction method.
in RNA isolation.
fluorescence in situ hybridization (FISH).
to dilute the samples of CVF (cervicovaginal fluid) and menstrual extracts for testing specificity through reverse transcriptase-polymerase chain reaction (RT-PCR) amplification of mRNA.
for DNA quantification using quantitative polymerase chain reaction (qPCR).

Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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A guide to transient expression of membrane proteins in HEK-293 cells for functional characterization

Ooi A, et al.

Frontiers in Physiology, 7, 300-300 (2016)

L1 retrotransposon expression in circulating tumor cells

Papasotiriou I, et al.

Testing, 12(2), e0171466-e0171466 (2017)

Registered report: Inhibition of BET recruitment to chromatin as an effective treatment for MLL-fusion leukemia

Fung J, et al.

eLife, 4, e08997-e08997 (2015)

Intracellular directed evolution of proteins from combinatorial libraries based on conditional phage replication.

Andreas K Brödel et al.

Nature protocols, 12(9), 1830-1843 (2017-08-11)

Directed evolution is a powerful tool to improve the characteristics of biomolecules. Here we present a protocol for the intracellular evolution of proteins with distinct differences and advantages in comparison with established techniques. These include the ability to select for

T1-Weighted Hypersignal in the Deep Cerebellar Nuclei After Repeated Administrations of Gadolinium-Based Contrast Agents in Healthy Rats: Difference Between Linear and Macrocyclic Agents.

Philippe Robert et al.

Investigative radiology, 50(8), 473-480 (2015-06-25)

To prospectively compare in healthy rats the effect of multiple injections of macrocyclic (gadoterate meglumine) and linear (gadodiamide) gadolinium-based contrast agents (GBCAs) on T1-weighted signal intensity in the deep cerebellar nuclei (DCN), including the dentate nucleus. Healthy rats (n =

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Global Trade Item Number

SKU	GTIN
95284-6X100ML	04061837895913
95284-100ML	04061837895890
95284-1L	04061837895906

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