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Merck
CN

96013

Abberior® STAR 635, maleimide

for STED application

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352200
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assay

≥80.0%

form

solid

solubility

DMF: 0.25 mg/mL, clear

fluorescence

λex 640 nm; λem 655 nm±10 nm in PBS, pH 7.4

storage temp.

−20°C

General description

Absorption Maximum, λmax: 635 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 110,000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.26 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.38 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 656 nm (aq. acetonitrile; MeOH)
655 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 750 −780 nm
Fluorescence Quantum Yield, η: 0.88 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 2.8 (PBS, pH 7.4)

Application

  • Abberior® Star 635 maleimide has been used for single-molecule FRET (Förster resonance energy transfer) spectroscopy for protein dynamics in live cells.
  • Abberior® STAR 635 goat anti-rabbit antibody has been used for STED (stimulated emission depletion) imaging of primary cultured striatal neurons obtained from rats.
  • Abberior® STAR 635 anti-rabbit antibody has been used for STED imaging of non-sensory supporting cells obtained from cochlea of rats and mice.
  • Abberior® STAR 635 conjugated with secondary antibody has been used for STED imaging of microtubules in cells.
Designed and tested for fluorescent super-resolution microscopy

Legal Information

abberior is a registered trademark of Abberior GmbH


Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Iwo König et al.
Nature methods, 12(8), 773-779 (2015-07-07)
Single-molecule methods have become widely used for quantifying the conformational heterogeneity and structural dynamics of biomolecules in vitro. Their application in vivo, however, has remained challenging owing to shortcomings in the design and reproducible delivery of labeled molecules, the range
Hans Blom et al.
PloS one, 8(9), e75155-e75155 (2013-09-24)
The phosphoprotein DARPP-32 (dopamine and cyclic adenosine 3´, 5´-monophosphate-regulated phosphoprotein, 32 kDa) is an important component in the molecular regulation of postsynaptic signaling in neostriatum. Despite the importance of this phosphoprotein, there is as yet little known about the nanoscale
Jenu Varghese Chacko et al.
Journal of biomedical optics, 19(10), 105003-105003 (2014-10-08)
Atomic force microscopes (AFM) provide topographical and mechanical information of the sample with very good axial resolution, but are limited in terms of chemical specificity and operation time-scale. An optical microscope coupled to an AFM can recognize and target an



Global Trade Item Number

SKUGTIN
96013-1MG04061823370455