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96924

Sigma-Aldrich

2,2,2-Trifluoroethanol

BioUltra, for molecular biology, ≥99.0% (GC)

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Synonym(s):
Trifluoroethyl alcohol
Linear Formula:
CF3CH2OH
CAS Number:
Molecular Weight:
100.04
Beilstein:
1733203
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

vapor density

3.5 (vs air)

vapor pressure

70 mmHg ( 25 °C)

product line

BioUltra

Assay

≥99.0% (GC)

form

liquid

autoignition temp.

~896 °F

expl. lim.

42 %

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

refractive index

n20/D 1.3 (lit.)

bp

77-80 °C (lit.)

mp

−44 °C (lit.)

density

1.373 g/mL at 25 °C (lit.)

cation traces

Al: ≤0.5 mg/kg
Ba: ≤0.1 mg/kg
Bi: ≤0.1 mg/kg
Ca: ≤0.5 mg/kg
Cd: ≤0.05 mg/kg
Co: ≤0.02 mg/kg
Cr: ≤0.02 mg/kg
Cu: ≤0.02 mg/kg
Fe: ≤0.1 mg/kg
K: ≤0.5 mg/kg
Li: ≤0.1 mg/kg
Mg: ≤0.1 mg/kg
Mn: ≤0.02 mg/kg
Mo: ≤0.1 mg/kg
Na: ≤1 mg/kg
Ni: ≤0.02 mg/kg
Pb: ≤0.1 mg/kg
Sr: ≤0.1 mg/kg
Zn: ≤0.1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: ≤0.03
λ: 280 nm Amax: ≤0.02

SMILES string

OCC(F)(F)F

application(s)

sample preparation

InChI

1S/C2H3F3O/c3-2(4,5)1-6/h6H,1H2

InChI key

RHQDFWAXVIIEBN-UHFFFAOYSA-N

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Application

2,2,2-Trifluoroethanol (Trifluoroethyl alcohol, TFE) is used to study the conformational states of proteins and the folding refolding processes of proteins.

Other Notes

Solvent used for sample preparation in electrophoresis

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Dam. 1 - Flam. Liq. 3 - Repr. 1B - STOT RE 2

Target Organs

Blood

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(C)

30 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

危险化学品

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T Rabilloud et al.
Electrophoresis, 19(6), 1006-1014 (1998-06-25)
Owing to the complexity of higher eukaryotic cells, characterization of a complete proteome is likely to be difficult to achieve. However, advantage can be taken of the cell compartmentalization to build organelle proteomes, which can moreover be viewed as specialized
K Shiraki et al.
Journal of molecular biology, 245(2), 180-194 (1995-01-13)
Trifluoroethanol (TFE) is known to stabilize the alpha-helical structure in proteins and their fragments. However, the relationship between the TFE-induced structures and the native structure is not clear. Here we show that beta-lactoglobulin, which consists predominantly of beta-sheets, exhibited a
Na Zhang et al.
Biomaterials science, 8(22), 6286-6300 (2020-10-07)
The regeneration of injured neurons over long injury distances remains suboptimal. In order to successfully stimulate nerve regrowth, potent biomolecules are necessary. Furthermore, reproducible and translatable methods to test the potency of candidate drugs for enhancing nerve regeneration over long
Bei Feng et al.
Biomacromolecules, 13(12), 3917-3925 (2012-11-08)
In tissue engineering research, there has recently been considerable interest in using electrospun biomimetic nanofibers of hybrids, in particular, from natural and synthetic polymers for engineering different tissues. However, phase separation between a pair of much dissimilar polymers might give
Kenichi Murai et al.
Organic & biomolecular chemistry, 11(16), 2648-2651 (2013-03-06)
CF3CH2OH was found to be a useful solvent for the oxidative rearrangement reactions of aminals promoted by N-chlorosuccinimide, which proceed via the intermediacy of in situ formed chloro-aminals and that produce 3,4-dihydroquinazolines.

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