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97875

Atto 647

Name: Atto 647
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥90% (HPCE)

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About This Item

UNSPSC Code:

12352108

NACRES:

NA.32

MDL number:

MFCD05865408

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product line

BioReagent

Quality Level

100

assay

≥90% (HPCE)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

transmittance

254 nm, 647 nm

fluorescence

λ_ex 643 nm; λ_em 665 nm in 0.1 M phosphate pH 7.0

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 645-651 nm A_max

suitability

suitable for fluorescence

storage temp.

−20°C

General description

Atto 647 is a zwitterionic dye with a net electrical charge of zero. It is a pH sensitive product. While practically stable up to pH 8.5, it slowly degrades at higher pH. Atto 647 is a new label with high molecular absorption (120,000) and quantum yield (0.20) as well as enough Stokes shift between excitation and emission maximum. Atto 647 is characterized by a high photostability and brightness. In addition, this label has good water solubility and low triplet formation ability.

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Cytokinesis,Volume 137 of Methods in Cell Biology (2017)

Novel disposable biochip platform employing supercritical angle fluorescence for enhanced fluorescence collection.

Duncan Hill et al.

Biomedical microdevices, 13(4), 759-767 (2011-05-12)

This paper presents an overview of development of a novel disposable plastic biochip for multiplexed clinical diagnostic applications. The disposable biochip is manufactured using a low-cost, rapid turn- around injection moulding process and consists of nine parabolic elements on a

Collagen containing microcapsules: smart containers for disease controlled therapy.

Laura Pastorino et al.

Journal of colloid and interface science, 357(1), 56-62 (2011-02-26)

The protein collagen is the major component of connective tissue and it is involved in many biological functions. Its degradation is at the basis of different pathological processes. The up-regulated expression of matrix metalloproteinases and the down-regulated expression of their

Stepwise loss of fluorescent core protein V from human adenovirus during entry into cells.

Daniel Puntener et al.

Journal of virology, 85(1), 481-496 (2010-11-05)

Human adenoviruses (Ads) replicate and assemble particles in the nucleus. They organize a linear double-strand DNA genome into a condensed core with about 180 nucleosomes, by the viral proteins VII (pVII), pX, and pV attaching the DNA to the capsid.

Pasteurella multocida toxin-stimulated osteoclast differentiation is B cell dependent.

Dagmar Hildebrand et al.

Infection and immunity, 79(1), 220-228 (2010-10-20)

Pasteurella multocida is a Gram-negative bacillus that infects a number of wild and domestic animals, causing respiratory diseases. Toxigenic Pasteurella multocida strains produce a protein toxin (PMT) that leads to atrophic rhinitis in swine due to enhanced osteoclastogenesis and the

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Global Trade Item Number

SKU	GTIN
97875-1MG-F	04061826749739

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