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D2650

Dimethyl sulfoxide

Hybri-Max™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

Synonym(s):

DMSO

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About This Item

Linear Formula:
(CH3)2SO
CAS Number:
67-68-5
Molecular Weight:
78.13
UNSPSC Code:
12191502
NACRES:
NA.75
PubChem Substance ID:
24893703
EC Number:
200-664-3
Beilstein/REAXYS Number:
506008
MDL number:
MFCD00002089
Assay:
≥99.7%
Grade:
Hybri-Max™
Technique(s):
cell culture | hybridoma: suitable, cryopreservation: suitable
Bp:
189 °C (lit.)
Vapor pressure:
0.42 mmHg ( 20 °C)
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grade

Hybri-Max™

Quality Level

400

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

sterility

sterile-filtered

product line

BioReagent

assay

≥99.7%

form

liquid

autoignition temp.

573 °F

feature

subscription eligible

expl. lim.

42 %, 63 °F

technique(s)

cell culture | hybridoma: suitable, cryopreservation: suitable

impurities

endotoxin, tested, ≤0.1% water
water

refractive index

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubility

H2O: miscible (completely)

density

1.10 g/mL (lit.)

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Application

DMSO is a polar aprotic solvent used in chemical reactions, in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal induced mechanical injury. It is used for frozen storage of primary, sub-cultured, and recombinant heteroploid and hybridoma cell lines; embryonic stem cells (ESC), and hematopoietic stem cells. DMSO is frequently used in the combinations with BSA or fetal bovine serum (FBS).

Packaging

This product features a liner in the cap designed to prevent leakage. In rare cases, the liner may remain on the neck of the bottle after cap removal. This does not affect the product′s integrity. If this occurs, simply reattach the liner to the cap.
100 mL in amber bottle
Glass ampoule packaging is now available under a new product number, D2653

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
This product is a Hybri-Max product. It is hybridoma tested and is assessed for suitability in cell freezing. This product is sterile filtered (0.2 micron) and tested for endotoxin levels.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Disclaimer

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

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Storage Class

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
RNBK5742
RNBK5429
RNBK5094
RNBK4887
RNBK4714

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Protocols

Cryopreservation of Cell Lines

Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.

Passaging Human iPS Cells Protocol Using EZ-LiFT™ Reagent

Human ips cell passaging protocol using EZ-LiFT™ Reagent, an enzyme-free and chemically defined pluripotent stem cell dissociation reagent.

Induced Pluripotent Stem Cell Culture Protocols

Step-by-step stem cell culture protocols for human induced pluripotent stem cells (iPSCs) including ips cell thawing, expanding, freezing and characterizing.

View All Protocols

Articles

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media

Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.

良好的细胞库操作实践

用于细胞系冷冻保存的良好细胞库操作实践概述。

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Materials science & engineering. C, Materials for biological applications, 103, 109767-109767 (2019-07-28)
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Cells, 8(7) (2019-07-25)
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Shady Sayed et al.
Methods (San Diego, Calif.), 164-165, 36-48 (2019-05-13)
The CRISPR/Cas9 system is transforming many biomedical disciplines, including cancer research. Through its flexible programmability and efficiency to induce DNA double strand breaks it has become straightforward to introduce cancer mutations into cells in vitro and/or in vivo. However, not
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Global Trade Item Number

SKUGTIN
D2650-5X5ML04061838447098
D2650-5X10ML04061838447081
D2650-100ML04061822705128