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Merck
CN

P3889

Polygalacturonic acid

≥85% (titration), from oranges

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About This Item

Linear Formula:
(C6H8O6)n
CAS Number:
25990-10-7
UNSPSC Code:
12352201
NACRES:
NA.25
MDL number:
MFCD00131972

Key Documents

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Product Name

Polygalacturonic acid, ≥85% (titration), from oranges

SMILES string

O1[C@@H]([C@@H]([C@H]([C@H]([C@H]1C(=O)O)O)O)O)O

InChI

1S/C6H10O7/c7-1-2(8)4(5(10)11)13-6(12)3(1)9/h1-4,6-9,12H,(H,10,11)/t1-,2+,3+,4-,6-/m0/s1

InChI key

AEMOLEFTQBMNLQ-BKBMJHBISA-N

biological source

oranges

assay

≥85% (titration)

form

powder

color

white to brown

mp

60 °C

solubility

aqueous NaOH: 1%, clear to hazy

storage temp.

room temp

Quality Level

200

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Application

Polygalacturonic acid has been used:
  • in the preabsorption of 2F4 primary antibody solution
  • as a substrate to determine polygalacturonase activity
  • in the preparation of oligogalacturonates mixture which is used as a substrate to determine the specificities of different acetylesterases

Polygalacturonic acid sequesters metal ions. It also produces gels in the presence of Ca2+ that can be used for drug release or to immobilize cells or enzymes for the production of biomolecules.

Biochem/physiol Actions

In land plants, polygalacturonic acids are major components of cell wall polysaccharides (pectins). Bound polygalacturonase-inhibiting peptides (PGIP) protect pectin from degradation by fungal polygalacturonases.

General description

Polygalacturonic acid is composed of galacturonic acid unit arranged as a long polymer chain. Methyl esters of polygalacturonic acid forms pectin.

Other Notes

To gain a comprehensive understanding of our extensive range of Polysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

植物油/植物胶产品
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Certificates of Analysis (COA)

Lot/Batch Number
BCCM5131
BCCK9237
BCCG7239
BCCJ3023
BCCD1273

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L Kurillová et al.
Journal of microencapsulation, 17(3), 279-296 (2000-05-20)
The structure of standard and stabilized calcium pectate gel (CPG) beads has been examined by scanning (SEM) and transmission (TEM) electron microscopy. A two-stage crosslinking procedure with polyethyleneimine (PEI) and glutaraldehyde (GA) led to the formation of a more compact
Biotechnology for Livestock Production, 246-246 (1989)
Mouyong Zou et al.
PloS one, 8(11), e79357-e79357 (2013-11-16)
An alkaline polygalacturonate lyase (PGL) from Bacillus subtilis 7-3-3, PelC, with diverse depolymerization abilities for different pectin substrates was found. The PGL activity of PelC decreased with increasing degree of methyl esterification of the substrate. PelA and PelC displayed notable
Zhangyong Dong et al.
BMC biochemistry, 12, 51-51 (2011-09-17)
Fusarium wilt is an economically devastating disease that affects banana production. Although Cavendish banana cultivars are resistant to Fusarium oxysporum f.sp. cubense race 1 (FOC1) and maitain banana production after Gros Michel was destructed by race 1, a new race
Bacterial Wilt Disease: Molecular and Ecological Aspects, 171-171 (1998)
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Protocols

Enzymatic Assay of Pectinase

To measure pectinase activity, a titrimetric stop reaction assay is used. One unit of pectinase will liberate 1 μmol of galacturonic acid from poly-galacturonic acid per hour at pH 4.0 at 25 °C.

果胶酶的酶促检测

在测定果胶酶活性时，使用了一种滴定终止反应法进行测定。在25 °C、pH 4.0条件下，一个单位的果胶酶每小时可从多聚半乳糖醛酸释放1 μmol 的半乳糖醛酸。

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