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P3889

Polygalacturonic acid

Name: Polygalacturonic acid
Brand: SIGMA

≥85% (titration), from oranges

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About This Item

Linear Formula:

(C₆H₈O₆)_n

CAS Number:

25990-10-7

UNSPSC Code:

12352201

NACRES:

NA.25

MDL number:

MFCD00131972

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Properties

biological source

oranges

Quality Level

200

assay

≥85% (titration)

form

powder

color

white to brown

mp

60 °C

solubility

aqueous NaOH: 1%, clear to hazy

storage temp.

room temp

SMILES string

O1[C@@H]([C@@H]([C@H]([C@H]([C@H]1C(=O)O)O)O)O)O

InChI

1S/C6H10O7/c7-1-2(8)4(5(10)11)13-6(12)3(1)9/h1-4,6-9,12H,(H,10,11)/t1-,2+,3+,4-,6-/m0/s1

InChI key

AEMOLEFTQBMNLQ-BKBMJHBISA-N

Description

General description

Polygalacturonic acid is composed of galacturonic acid unit arranged as a long polymer chain. Methyl esters of polygalacturonic acid forms pectin.

Application

Polygalacturonic acid has been used:

in the preabsorption of 2F4 primary antibody solution
as a substrate to determine polygalacturonase activity
in the preparation of oligogalacturonates mixture which is used as a substrate to determine the specificities of different acetylesterases

Polygalacturonic acid sequesters metal ions. It also produces gels in the presence of Ca²⁺ that can be used for drug release or to immobilize cells or enzymes for the production of biomolecules.

Biochem/physiol Actions

In land plants, polygalacturonic acids are major components of cell wall polysaccharides (pectins). Bound polygalacturonase-inhibiting peptides (PGIP) protect pectin from degradation by fungal polygalacturonases.

Other Notes

To gain a comprehensive understanding of our extensive range of Polysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

植物油/植物胶产品

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Protocols

Enzymatic Assay of Pectinase

To measure pectinase activity, a titrimetric stop reaction assay is used. One unit of pectinase will liberate 1 μmol of galacturonic acid from poly-galacturonic acid per hour at pH 4.0 at 25 °C.

果胶酶的酶促检测

在测定果胶酶活性时，使用了一种滴定终止反应法进行测定。在25 °C、pH 4.0条件下，一个单位的果胶酶每小时可从多聚半乳糖醛酸释放1 μmol 的半乳糖醛酸。

Bacterial Wilt Disease: Molecular and Ecological Aspects, 171-171 (1998)

Isolation and characterization of an exopolygalacturonase from Fusarium oxysporum f.sp. cubense race 1 and race 4.

Zhangyong Dong et al.

BMC biochemistry, 12, 51-51 (2011-09-17)

Fusarium wilt is an economically devastating disease that affects banana production. Although Cavendish banana cultivars are resistant to Fusarium oxysporum f.sp. cubense race 1 (FOC1) and maitain banana production after Gros Michel was destructed by race 1, a new race

Pectin acetylesterases from Aspergillus are able to deacetylate homogalacturonan as well as rhamnogalacturonan

Bonnin E, et al.

Carbohydrate Polymers, 74(3), 411-418 (2008)

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Global Trade Item Number

SKU	GTIN
P3889-25G	04061834373193
P3889-5G	04061834373209
P3889-500G	04061832630335
P3889-100G	04061834373186