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P9172

Pyronin Y

Name: Pyronin Y
Brand: SIGMA

for NA electrophoresis

Synonym(s):

Pyronin Y, Pyronin G, Pyronin J

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About This Item

Empirical Formula (Hill Notation):

C₁₇H₁₉ClN₂O

CAS Number:

92-32-0

Molecular Weight:

302.80

EC Number:

202-147-8

UNSPSC Code:

12171500

NACRES:

NA.52

PubChem Substance ID:

57654569

MDL number:

MFCD00011725

Beilstein/REAXYS Number:

3795789

Colour Index Number:

45005

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Properties

grade

Molecular Biology

Quality Level

200

form

powder

composition

Dye content, ≥45%

technique(s)

nucleic acid detection: suitable

mp

250-260 °C (lit.)

solubility

water: 10 mg/mL, dark red

suitability

suitable for nucleic acid staining

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc2C=C3C=C\C(C=C3Oc2c1)=[N+](/C)C

InChI

1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1

InChI key

INCIMLINXXICKS-UHFFFAOYSA-M

Description

Application

Pyronin Y has been used:

in the purification of quiescent and cycling hematopoietic stem cells and staining of bone marrow cells (HSCs)
as a component of the polychlorinated biphenyls (PCBS) solution for staining mammary gland side population cells (MG-SPs)
staining of CD34⁺ cells

Biochem/physiol Actions

Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells. In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections.

Preparation Note

This product is soluble in water 1 mg/ml, yielding a clear dark red solution.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Global Trade Item Number

SKU	GTIN
P9172-1G	04061834402572
P9172-5G	04061834402589