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P9172

Pyronin Y

Name: Pyronin Y
Brand: SIGMA

for NA electrophoresis

Synonym(s):

Pyronin Y, Pyronin G, Pyronin J

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About This Item

Empirical Formula (Hill Notation):

C₁₇H₁₉ClN₂O

CAS Number:

92-32-0

Molecular Weight:

302.80

EC Number:

202-147-8

UNSPSC Code:

12171500

NACRES:

NA.52

PubChem Substance ID:

57654569

MDL number:

MFCD00011725

Beilstein/REAXYS Number:

3795789

Colour Index Number:

45005

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grade

Molecular Biology

Quality Level

200

form

powder

composition

Dye content, ≥45%

technique(s)

nucleic acid detection: suitable

mp

250-260 °C (lit.)

solubility

water: 10 mg/mL, dark red

suitability

suitable for nucleic acid staining

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc2C=C3C=C\C(C=C3Oc2c1)=[N+](/C)C

InChI

1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1

InChI key

INCIMLINXXICKS-UHFFFAOYSA-M

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Application

Pyronin Y has been used:

in the purification of quiescent and cycling hematopoietic stem cells and staining of bone marrow cells (HSCs)
as a component of the polychlorinated biphenyls (PCBS) solution for staining mammary gland side population cells (MG-SPs)
staining of CD34⁺ cells

Biochem/physiol Actions

Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells. In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections.

Preparation Note

This product is soluble in water 1 mg/ml, yielding a clear dark red solution.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Photosensitizing effects of the tricyclic heteroaromatic cationic dyes pyronin Y and toluidine blue O (tolonium chloride

Darzynkiewicz Z and Carter SP

Cancer research, 48(5) (1988)

Staining of RNA after polyacrylamide gel electrophoresis.

K Marcinka

Methods in cell biology, 10, 49-68 (1975-01-01)

Gene expression differences between enriched normal and chronic myelogenous leukemia quiescent stem/progenitor cells and correlations with biological abnormalities

Affer M, et al.

Journal of Oncology, 2011 (2011)

Transcriptional profiling of mammary gland side population cells

Behbod F, et al.

Stem Cells, 24(4), 1065-1074 (2006)

Senescence and apoptosis block hematopoietic activation of quiescent hematopoietic stem cells with short telomeres

Wang J, et al.

Blood, 124(22), 3237-3240 (2014)

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Global Trade Item Number

SKU	GTIN
P9172-1G	04061834402572
P9172-5G	04061834402589

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