RDRT
ReadyScript® cDNA Synthesis Mix
Complete reagent for first strand cDNA synthesis for RT-qPCR
Synonym(s):
cDNA Synthesis Mix
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About This Item
Recommended Products
usage
sufficient for 100 reactions
sufficient for 25 reactions
sufficient for 500 reactions
feature
dNTPs included
hotstart: no
technique(s)
RT-PCR: suitable
color
colorless
input
purified RNA
shipped in
dry ice
storage temp.
−20°C
1 of 4
This Item | P4600 | MIRRT | 11483188001 |
---|---|---|---|
technique(s) RT-PCR: suitable | technique(s) PCR: suitable | technique(s) cDNA synthesis: suitable | technique(s) RT-PCR: suitable |
usage sufficient for 100 reactions | usage sufficient for 100 reactions | usage - | usage sufficient for 30 reactions (including 5 control reactions) |
feature dNTPs included | feature dNTPs included, hotstart: no | feature - | feature dNTPs included, hotstart: no |
color colorless | color colorless | color - | color - |
input purified RNA | input purified DNA | input - | input purified RNA |
shipped in dry ice | shipped in wet ice | shipped in wet ice | shipped in - |
General description
Application
Features and Benefits
- Convenient 5X master mix formula provides for quick and easy reaction set up; just add RNA template
- Includes proprietary blend of randomers and oligodT primers and an RNAse H+ derivative of Moloney murine leukemia virus (MMLV) reverse transcriptase
- Allows for unbiased, accurate conversion of RNA to cDNA in just 40 minutes
- Can use up to 1 μg of RNA in a single 20 μL reaction; reactions can also be scaled up
- Highly stable; can be stored at -20°C for up to 1 year; 4°C for up to one month
- Specific ratio of randomers and oligo (dT) primers in the mastermix ensures optimal representation of all transcript sequences in the cDNA product
- High reproducibility: Premixed master mix eliminates variability inherent in other kits requiring mixing of components
- RNase H(+) Moloney murine leukemia virus (MMLV) reverse transcriptase ensures residual RNA does not interfere with PCR amplification
- Reverse transcribes both rare and abundant genes with a wide input range of template
- Convenient 5X master mix formula provides for quick and easy reaction set up; just add RNA template
Other Notes
Legal Information
related product
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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