RDRT
ReadyScript® cDNA Synthesis Mix
Complete reagent for first strand cDNA synthesis for RT-qPCR
Synonym(s):
cDNA Synthesis Mix
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About This Item
usage
sufficient for 100 reactions, sufficient for 25 reactions, sufficient for 500 reactions
feature
dNTPs included, hotstart: no
technique(s)
cDNA synthesis: suitable, RT-PCR: suitable
color
colorless
input
purified RNA
shipped in
dry ice
storage temp.
−20°C
Related Categories
General description
Application
Features and Benefits
- Convenient 5X master mix formula provides for quick and easy reaction set up; just add RNA template
- Includes proprietary blend of randomers and oligodT primers and an RNAse H+ derivative of Moloney murine leukemia virus (MMLV) reverse transcriptase
- Allows for unbiased, accurate conversion of RNA to cDNA in just 40 minutes
- Can use up to 1 μg of RNA in a single 20 μL reaction; reactions can also be scaled up
- Highly stable; can be stored at -20°C for up to 1 year; 4°C for up to one month
- Specific ratio of randomers and oligo (dT) primers in the mastermix ensures optimal representation of all transcript sequences in the cDNA product
- High reproducibility: Premixed master mix eliminates variability inherent in other kits requiring mixing of components
- RNase H(+) Moloney murine leukemia virus (MMLV) reverse transcriptase ensures residual RNA does not interfere with PCR amplification
- Reverse transcribes both rare and abundant genes with a wide input range of template
- Convenient 5X master mix formula provides for quick and easy reaction set up; just add RNA template
Other Notes
Legal Information
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Articles
Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.
Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.
Protocols
Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.
Reverse transcription analyzes mRNA gene expression, facing challenges like transcript half-life differences and temporal patterns.
Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions
将RNA逆转录成DNA的方法。使用含有逆转录酶、dNTP、引物、RNase抑制剂和缓冲液的预混试剂。快速合成cDNA。
Related Content
PCR是分子生物学的核心技术，功能强大。可在体外高效快速地对不同来源的特定DNA或RNA序列进行酶扩增。
RT-qPCR detects specific targets with applications in gene expression and pathogen detection.
Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| RDRT-500RXN | 04061835514014 |
| RDRT-25RXN | 04061835574834 |
| RDRT-100RXN | 04061835514007 |
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