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Merck
CN

A0262

Lithium potassium acetyl phosphate

≥85% (elemental analysis), powder

Synonym(s):

Acetyl phosphate lithium potassium salt

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About This Item

Linear Formula:
CH3COOP(O)(OK)(OLi)
CAS Number:
94249-01-1
Molecular Weight:
184.06
NACRES:
NA.32
PubChem Substance ID:
24890415
UNSPSC Code:
12352204
EC Number:
304-378-0
MDL number:
MFCD00002134
Beilstein/REAXYS Number:
5689718

Key Documents

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Product Name

Lithium potassium acetyl phosphate, high-energy phosphate donor

InChI

1S/C2H5O5P.K.Li/c1-2(3)7-8(4,5)6;;/h1H3,(H2,4,5,6);;/q;2*+1/p-2

InChI key

RLQMPLKXFIXRCV-UHFFFAOYSA-L

SMILES string

[Li+].[K+].CC(=O)OP([O-])([O-])=O

assay

≥85% (elemental analysis)

form

powder

mp

>300 °C (lit.)

solubility

water: 25 mg/mL, clear, colorless

storage temp.

−20°C

Quality Level

200

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Application

Lithium potassium acetyl phosphate:
  • has been used as a substrate for CpxR (transcription factor) in x-ray crystallography to soak CpxR receiver domain (CpxRRD) crystals to obtain the phosphorylated form
  • may be used as a phosphate (P) donor in a study to screen a wide range of natural and synthetic organic phosphate donors with several enzymes on various hydroxyl?compounds for the improved enzymatic synthesis of phosphate monoesters
  • has been used to set up premixA for in vitro translation reaction

Biochem/physiol Actions

Lithium potassium acetyl phosphate is commonly used as the source for acetyl phosphate. So acetyl phosphate can be used as a suitable alternative to replace inorganic oligophosphates in phosphatase-catalyzed transphosphorylation to reduce inorganic monophosphate by-product formation.

pictograms

Health hazardCorrosion
GHS08,GHS05

signalword

Danger

hcodes

H314,H361

Hazard Classifications

Eye Dam. 1 - Repr. 2 - Skin Corr. 1B

Storage Class

8B - Non-combustible corrosive hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN3844
BCCM7976
057K5317V
057K5317
BCBC3616V

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Ariel E Mechaly et al.
Structure (London, England : 1993), 25(6), 939-944 (2017-05-30)
Bacterial two-component systems consist of a sensor histidine kinase (HK) and a response regulator (RR). HKs are homodimers that catalyze the autophosphorylation of a histidine residue and the subsequent phosphoryl transfer to its RR partner, triggering an adaptive response. How
Soma Mukhopadhyay et al.
Bioorganic chemistry, 36(2), 65-69 (2008-02-26)
Acetate kinase, a member of the ASKHA (Acetate and Sugar Kinases, Hsp70, Actin) phosphotransferase superfamily is a central enzyme in prokaryotic carbon and energy metabolism. Recently extensive structural and biochemical studies of acetate kinase and related carboxylate kinases have been
Gábor Tasnádi et al.
Advanced synthesis & catalysis, 360(12), 2394-2401 (2018-10-20)
Undesired product hydrolysis along with large amounts of waste in form of inorganic monophosphate by-product are the main obstacles associated with the use of pyrophosphate in the phosphatase-catalyzed synthesis of phosphate monoesters on large scale. In order to overcome both
Sebastian R Schmidl et al.
Nature chemical biology, 15(7), 690-698 (2019-05-22)
Two-component systems (TCSs) are the largest family of multi-step signal transduction pathways and valuable sensors for synthetic biology. However, most TCSs remain uncharacterized or difficult to harness for applications. Major challenges are that many TCS output promoters are unknown, subject
Birgit Dreier et al.
Methods in molecular biology (Clifton, N.J.), 687, 283-306 (2010-10-23)
The selection and concomitant affinity maturation of proteins to bind to user-defined target molecules have become a key technology in biochemical research, diagnostics, and therapy. One of the most potent selection technologies for such applications is ribosome display. It works
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